Pseudomonas toxin has been coupled to monoclonal antibodies to make immunotoxins. When coupled to an antibody to the T cell growth factor receptor to make anti-TAC-PE, it kills leukemia cells that are TAC positive. When injected into monkeys, the immunotoxin has very little toxicity and good survival indicating it may be a useful agent for treatment of this type of leukemia. Adenovirus enters cells in the same vesicles (receptosomes) as these immunotoxins. By lysing receptosomes, adenovirus efficiently releases the immunotoxin into the cytosol and selectively increases cell killing. The penton base of adenovirus is important for vesicle lysis. Adenovirus at pH 5 will permeabilize the plasma membrane of KB cells to small molecules.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Biology And Diagnosis (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB008753-03
Application #
4691876
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code