The objective of this project is to understand the molecular mechanisms by which genes for connective tissue proteins are regulated and expressed during normal development and in disease states. The formation of extracellular matrix is intimately associated with cell differentiation and tissue development in mammalian embryogenesis. Furthermore, alterations of extracellular matrix structure are associated with human conditions such as neoplastic disease and diabetes. We are studying genetic mechanisms controlling the expression and function of cartilage and basement membrane components. Recombinant DNA techniques have been used to prepared molecular clones of genes and mRNA for various cartilage and basement membrane constituents. Using these molecular tools, the genetic mechanism(s) controlling the expression of the genes has been studied. The structure of some of these constituents has been determined by cDNA sequencing. Relationships between the structure and function of the proteins have been studied using synthetic peptides deduced from cDNA sequence. Sequences in these genes regulating their expression are being studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000025-22
Application #
3939892
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
22
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code