Abstract

Renal disease is difficult to detect, particularly in a form called acute renal failure. We are developing new methods to detect renal disease involving either MRI, or urine or blood tests. 1)Detection of proximal tubule damage in rats MRI using G4 dendrimer gadolinium chelate (submitted, Kidney International). 2) Is inflammation involved in ARF? MRI using ultra-small iron oxide particles (USPIO) for inflammation 3) Markers for early diagnosis We have seached for new genes that are up-regulated following injury in humans and animals. One of the markers (RDT001; Provisional patent in progress; MS in preparation) is upregulated in kidney tissue, and in preliminary experiments, also upregulated in blood and urine. We are making a quantitative assay for RDT001. We will soon test it on human samples (some we have collected, some from 2-3 collaborating groups outside NIH).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Intramural Research (Z01)
Project #
1Z01DK043400-02
Application #
6535223
Study Section
(MDB)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Doi, Kent; Yuen, Peter S T; Eisner, Christoph et al. (2009) Reduced production of creatinine limits its use as marker of kidney injury in sepsis. J Am Soc Nephrol 20:1217-21
Zhou, Hua; Cheruvanky, Anita; Hu, Xuzhen et al. (2008) Urinary exosomal transcription factors, a new class of biomarkers for renal disease. Kidney Int 74:613-21
Bennett, Kevin M; Zhou, Hua; Sumner, James P et al. (2008) MRI of the basement membrane using charged nanoparticles as contrast agents. Magn Reson Med 60:564-74
Dear, J W; Yuen, P S T (2008) Setting the stage for acute-on-chronic kidney injury. Kidney Int 74:7-9
Dear, James W; Leelahavanichkul, Asada; Aponte, Angel et al. (2007) Liver proteomics for therapeutic drug discovery: inhibition of the cyclophilin receptor CD147 attenuates sepsis-induced acute renal failure. Crit Care Med 35:2319-28
Cheruvanky, Anita; Zhou, Hua; Pisitkun, Trairak et al. (2007) Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator. Am J Physiol Renal Physiol 292:F1657-61
Zhou, Hua; Hewitt, Stephen M; Yuen, Peter S T et al. (2006) Acute Kidney Injury Biomarkers - Needs, Present Status, and Future Promise. Nephrol Self Assess Program 5:63-71
Zhou, H; Yuen, P S T; Pisitkun, T et al. (2006) Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int 69:1471-6
Holly, M K; Dear, J W; Hu, X et al. (2006) Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure. Kidney Int 70:496-506
Zhou, H; Pisitkun, T; Aponte, A et al. (2006) Exosomal Fetuin-A identified by proteomics: a novel urinary biomarker for detecting acute kidney injury. Kidney Int 70:1847-57

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