Abstract

Renal disease is difficult to detect, particularly in a form called acute renal failure. We are developing new methods to detect renal disease involving either urine or blood tests. We have seached for new genes that are up-regulated following injury in humans and animals. We had hoped that some of these genes would be good markers of disease. Unfortunately, these did not work out. We have started searching in serum using proteomic techniques. We have some promising preliminary results for a new blood test for a certain form of renal disease, but need to repeat the study.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Intramural Research (Z01)
Project #
1Z01DK043400-01
Application #
6421380
Study Section
(MDB)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Doi, Kent; Yuen, Peter S T; Eisner, Christoph et al. (2009) Reduced production of creatinine limits its use as marker of kidney injury in sepsis. J Am Soc Nephrol 20:1217-21
Zhou, Hua; Cheruvanky, Anita; Hu, Xuzhen et al. (2008) Urinary exosomal transcription factors, a new class of biomarkers for renal disease. Kidney Int 74:613-21
Bennett, Kevin M; Zhou, Hua; Sumner, James P et al. (2008) MRI of the basement membrane using charged nanoparticles as contrast agents. Magn Reson Med 60:564-74
Dear, J W; Yuen, P S T (2008) Setting the stage for acute-on-chronic kidney injury. Kidney Int 74:7-9
Dear, James W; Leelahavanichkul, Asada; Aponte, Angel et al. (2007) Liver proteomics for therapeutic drug discovery: inhibition of the cyclophilin receptor CD147 attenuates sepsis-induced acute renal failure. Crit Care Med 35:2319-28
Cheruvanky, Anita; Zhou, Hua; Pisitkun, Trairak et al. (2007) Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator. Am J Physiol Renal Physiol 292:F1657-61
Zhou, Hua; Hewitt, Stephen M; Yuen, Peter S T et al. (2006) Acute Kidney Injury Biomarkers - Needs, Present Status, and Future Promise. Nephrol Self Assess Program 5:63-71
Zhou, H; Yuen, P S T; Pisitkun, T et al. (2006) Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int 69:1471-6
Holly, M K; Dear, J W; Hu, X et al. (2006) Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure. Kidney Int 70:496-506
Zhou, H; Pisitkun, T; Aponte, A et al. (2006) Exosomal Fetuin-A identified by proteomics: a novel urinary biomarker for detecting acute kidney injury. Kidney Int 70:1847-57

Showing the most recent 10 out of 35 publications