Using immunohistochemical techniques in combination with retrograde tracing, we have examined the innervating transmitters of several brainstem nuclei, including peptides, catecholamines and indoleamines. As a continuation of our interest in the hypothalamus, we have gathered evidence on an NPY-adrenaline projection from the brainstem to the paraventricular nucleus (PVN). Evidence has also been obtained that a subset of the corticotropin releasing factor (CRF) neurons in the PVN coexist with cholecystokinin (CCK). In addition, VIP projections in the hypothalamus have been examined with regard to projections from the suprachiasmatic nucleus to the median eminence. In another series of studies, we have confirmed a coexisting Substance P-serotonin projection to one area responsible for central respiratory control, the phrenic motor nucleus. In dorsal root ganglia, evidence has been obtained to suggest co-staining of a subpopulation of neurons with both carbonic anyhydrase and cholera toxin. Finally, ongoing studies are exploring: the coexistence of dynorphin and GABA; a complete mapping of the stria terminalis; and cholinergic projections to the mesencephalon.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH000179-04
Application #
4696314
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code