We have been developing several new anatomical methods to enhance our studies of coexisting transmitters in the brain. These include intensification of immunohistochemistry, double and triple labeling of single brain sections, new retrograde dyes such as fluorogold and in situ hybridization. In addition, we have been using these techniques to examine the effects of adrenalectomy on neuropeptide (dynorphin and vasopressin) in the hypothalamus and innervation of the phrenic motor nucleus. Finally, studies using antibodies specific to a subpopulation of dorsal root ganglion cells and studies tracing cholinergic projections to both substantia nigra and prefrontal cortex are underway.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH000179-05
Application #
3968411
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code