Abstract

Research on various aspects of Giardia lamblia is limited. Most studies are collaborative in nature. Using a model of Giardia infection developed in this lab and based on earlier studies performed at NIH, Steve Singer's and his lab show that Il-6, which is important in control of infection in mice is not produced by T cells but by dendritic cells. We have completed genomic sequencing of isolates GS( genotype B) and DH( genotype A2) and compared theme to a draft sequence of GS and the previously published sequence of WB. THe results show that GS is very different from WB and DH and strongly suggests it is a different species.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI000161-35
Application #
8555735
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
35
Fiscal Year
2012
Total Cost
$227,688
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Nash, Theodore E (2013) Unraveling how Giardia infections cause disease. J Clin Invest 123:2346-7
Adam, Rodney D; Dahlstrom, Eric W; Martens, Craig A et al. (2013) Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig). Genome Biol Evol 5:2498-511
Kamda, Joel D; Nash, Theodore E; Singer, Steven M (2012) Giardia duodenalis: dendritic cell defects in IL-6 deficient mice contribute to susceptibility to intestinal infection. Exp Parasitol 130:288-91
Ramanathan, Roshan; Talaat, Kawsar R; Fedorko, Daniel P et al. (2011) A species-specific approach to the use of non-antimony treatments for cutaneous leishmaniasis. Am J Trop Med Hyg 84:109-17
Adam, Rodney D; Nigam, Anuranjini; Seshadri, Vishwas et al. (2010) The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. BMC Genomics 11:424
Galkin, Andrey; Kulakova, Liudmila; Wu, Rui et al. (2010) X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia. Acta Crystallogr Sect F Struct Biol Cryst Commun 66:386-90
Burneo, Jorge G; Del Brutto, Oscar; Delgado-Escueta, Antonio V et al. (2009) Workshop report: Developing an international collaborative research network in neurocysticercosis and epilepsy. Epilepsia 50:1289-90
Li, Zhimin; Kulakova, Liudmila; Li, Ling et al. (2009) Mechanisms of catalysis and inhibition operative in the arginine deiminase from the human pathogen Giardia lamblia. Bioorg Chem 37:149-61
Elias, Eliana V; Quiroga, Rodrigo; Gottig, Natalia et al. (2008) Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia. J Biol Chem 283:35996-6010
Touz, Maria Carolina; Ropolo, Andrea Silvana; Rivero, Maria Romina et al. (2008) Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia. J Cell Sci 121:2930-8