Abstract

1. Adult supporting cell transcriptome: we are in the process of characterizing and validating transcriptome (single-cell mRNA-Seq) findings in the adult mammalian cochlea in both mouse and human temporal bone tissue. 2. Mechanisms of endocochlear potential development and degeneration in the mammalian organ of Corti: accumulation of cDNA from single cells in the stria vascularis and lateral wall during endocochlear potential development and into maturity for single cell mRNA-Seq profiling is underway. We are investigating the potential of novel imaging modalities to characterize the cell surface interactions between marginal and intermediate cells in the stria vascularis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADC000088-01
Application #
9147442
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost

  Institution

Name
Deafness & Other Communication Disorders
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Sharlin, David S; Ng, Lily; Verrey, François et al. (2018) Deafness and loss of cochlear hair cells in the absence of thyroid hormone transporters Slc16a2 (Mct8) and Slc16a10 (Mct10). Sci Rep 8:4403
Imtiaz, Ayesha; Belyantseva, Inna A; Beirl, Alisha J et al. (2018) CDC14A phosphatase is essential for hearing and male fertility in mouse and human. Hum Mol Genet 27:780-798
Honda, Keiji; Kim, Sung Huhn; Kelly, Michael C et al. (2017) Molecular architecture underlying fluid absorption by the developing inner ear. Elife 6:
Nakanishi, Hiroshi; Kawashima, Yoshiyuki; Kurima, Kiyoto et al. (2017) NLRP3 mutation and cochlear autoinflammation cause syndromic and nonsyndromic hearing loss DFNA34 responsive to anakinra therapy. Proc Natl Acad Sci U S A 114:E7766-E7775
Burns, Joseph C; Kelly, Michael C; Hoa, Michael et al. (2015) Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun 6:8557
Hoa, Michael; Friedman, Rick A; Fisher, Laurel M et al. (2015) Prognostic implications of and audiometric evidence for hearing fluctuation in Meniere's disease. Laryngoscope :
Dhillon, Vaninder K; Hoa, Michael; Winter, Margaret et al. (2014) Extrusion of a cochlear implant positioner through the tympanic membrane in a pediatric patient: management of a delayed complication. Ann Otol Rhinol Laryngol 123:537-40
Hoa, Michael; Wilkinson, Eric P; Slattery 3rd, William H (2014) Delayed recovery of speech discrimination after fractionated stereotactic radiotherapy for vestibular schwannoma in neurofibromatosis 2. Ear Nose Throat J 93:E20-2