Researchers from 11 Sections/ Laboratories/ Branches have used the Facility during the past year. Publications resulting from this work are listed in the bibliography. Several manuscripts have been submitted recently and are not listed. Microscopy done in LIS in the past year has contributed to expand knowledge in each of the NIAMS research areas: i) understanding the immune system and its diseases (Casellas et al, 2009); ii) understanding bone and cartilage, their multipotent cells and possible modes of repair, as well as bioengineering of replacement tissues (Colburn et al, 2009;Gagarina et al, 2010;Pricola et al, 2009); iii) understanding skin development (Duverger &Morasso, 2009); iv) understanding basic cardiac (Manisastry et al, 2009) and skeletal (Zhang et al, 2009) muscle biologies; v) understanding muscle diseases such as Pompe Disease (Raben et al., 2009 &2010;Takikita et al, 2009;Xu et al, 2010) and Duchenne Muscular Dystrophy (Prins et al, 2009). The reach of the microscopy techniques available to NIAMS researchers has been expanded by several additions: a laser capture and microdissection instrument has been upgraded and moved to the Facility;several software and hardware improvements have been made to the Total Internal Reflection Fluorescence (TIRF) microscopy system;and thanks to ARRA funds we have added a single molecule detection platform for Fluorescence Lifetime Imaging Microscopy (FLIM). Finally, one of our confocal microscopes has been fitted with an objective inverter, new objective and external stage for 2-photon intravital microscopy.

Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2010
Total Cost
$875,826
Indirect Cost
Name
National Institute of Arthritis and Musculoskeletal and Skin Diseases
Department
Type
DUNS #
City
State
Country
Zip Code
Iglesias-Bartolome, Ramiro; Uchiyama, Akihiko; Molinolo, Alfredo A et al. (2018) Transcriptional signature primes human oral mucosa for rapid wound healing. Sci Transl Med 10:
Sarshad, Aishe A; Juan, Aster H; Muler, Ana Iris Correa et al. (2018) Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells. Mol Cell :
Duverger, Olivier; Carlson, Jenna C; Karacz, Chelsea M et al. (2018) Genetic variants in pachyonychia congenita-associated keratins increase susceptibility to tooth decay. PLoS Genet 14:e1007168
Feng, Li Rebekah; Fernández-Martínez, Juan Luis; Zaal, Kristien J M et al. (2018) mGluR5 mediates post-radiotherapy fatigue development in cancer patients. Transl Psychiatry 8:110
Lee, Jeansun; Dieckmann, Nele M G; Edgar, James R et al. (2018) Fas Ligand localizes to intraluminal vesicles within NK cell cytolytic granules and is enriched at the immune synapse. Immun Inflamm Dis 6:312-321
Wolf, M; Ao, M; Chavez, M B et al. (2018) Reduced Orthodontic Tooth Movement in Enpp1 Mutant Mice with Hypercementosis. J Dent Res 97:937-945
Gupta, Sarthak; Chan, Diana W; Zaal, Kristien J et al. (2018) A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils. J Immunol 200:869-879
Thumbigere Math, V; Rebouças, P; Giovani, P A et al. (2018) Periodontitis in Chédiak-Higashi Syndrome: An Altered Immunoinflammatory Response. JDR Clin Trans Res 3:35-46
Bhattacharya, Shreya; Kim, Jin-Chul; Ogawa, Youichi et al. (2018) DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis. J Invest Dermatol 138:1052-1061
Sikora, Keith A; Bennett, Joshua R; Vyncke, Laurens et al. (2018) Germline gain-of-function myeloid differentiation primary response gene-88 (MYD88) mutation in a child with severe arthritis. J Allergy Clin Immunol 141:1943-1947.e9

Showing the most recent 10 out of 81 publications