The heterogeneous nature of tissue has proven to be the limiting factor in the amount of biological information that can be generated from tissue samples. LCM provides the researcher with a means to isolate pure population of cells from heterogeneous tissue specimens. These """"""""captured"""""""" cells can then be used in a wide range of downstream assays such as LOH (loss of heterozygosity) studies, gene expression analysis at the mRNA level or in a wide range of proteomic assays such as 2D gel analysis, Western blotting, reverse phase protein array and SELDI protein profiling. Commercial kits for the isolation of RNA and DNA are available and adaptable to the micro samples obtained by LCM.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC011068-03
Application #
8158361
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2010
Total Cost
$248,687
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
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Erickson, Heidi S; Albert, Paul S; Gillespie, John W et al. (2009) Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples. Nat Protoc 4:902-22