The heterogeneous nature of tissue has proven to be the limiting factor in the amount of biological information that can be generated from tissue samples. LCM provides the researcher with a means to isolate pure population of cells from heterogeneous tissue specimens. These """"""""captured"""""""" cells can then be used in a wide range of downstream assays such as LOH (loss of heterozygosity) studies, gene expression analysis at the mRNA level or in a wide range of proteomic assays such as 2D gel analysis, Western blotting, reverse phase protein array and SELDI protein profiling. Commercial kits for the isolation of RNA and DNA are available and adaptable to the micro samples obtained by LCM.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Scientific Cores Intramural Research (ZIC)
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National Cancer Institute Division of Basic Sciences
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Roy Chowdhuri, Sinchita; Hanson, Jeffrey; Cheng, Jerome et al. (2012) Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples. Acta Cytol 56:622-31
Hipp, Jason; Monaco, James; Kunju, L Priya et al. (2012) Integration of architectural and cytologic driven image algorithms for prostate adenocarcinoma identification. Anal Cell Pathol (Amst) 35:251-65
Eberle, Franziska C; Hanson, Jeffrey C; Killian, J Keith et al. (2010) Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens. J Mol Diagn 12:394-401
Armani, Michael; Rodriguez-Canales, Jaime; Gillespie, John et al. (2009) 2D-PCR: a method of mapping DNA in tissue sections. Lab Chip 9:3526-34
Erickson, Heidi S; Albert, Paul S; Gillespie, John W et al. (2009) Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples. Nat Protoc 4:902-22