Abstract

A significant portion of the Labs activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs around 100 processes per year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 200 liters), and recovery and purification of biomolecules, such as proteins and polysaccharides, from different sources. The following are examples of processes performed: growth of bacteria like Escherichia coli, (recombinant and native), Salmonella, Shighella, Staphylococcus, Haemophilus influenzae, N. meningitidis (meningococcus), Borrelia burgdorferi, Halobacteria, Bronchaseptica sp, Micobacteria sp, and various recombinant yeast strains like Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, MDCK and HEK 293, and insect cell such Sf 9, and High five for transient expression of recombinant proteins were propagated. Several proteins were produced and purified in gram quantities. Examples are: recombinant Clostridium difficle toxin A, recombinant human adiponectin, recombinant pseudomonas aeruginasa exotoxin A, recombinant Diphtheria toxin and recombinant Plasmodium proteins for malaria vaccine research. The various products were needed for a number of collaborative research projects: human adiponectin was made for biological studies, the bacterial toxins for vaccine development and several proteins were produced for structural studies among them Toll proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICDK015500-51
Application #
8554134
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
51
Fiscal Year
2012
Total Cost
$631,190
Indirect Cost

  Institution

Name
National Institute of Diabetes and Digestive and Kidney Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Mulangu, Sabue; Alfonso, Vivian H; Hoff, Nicole A et al. (2018) Serologic Evidence of Ebolavirus Infection in a Population With No History of Outbreaks in the Democratic Republic of the Congo. J Infect Dis 217:529-537
Inwood, Sarah; Xu, Hui; Black, Mary A et al. (2018) Continuous production process of retroviral vector for adoptive T- cell therapy. Biochem Eng J 132:145-151
Qiang, Xiaoling; Liotta, Anthony S; Shiloach, Joseph et al. (2017) New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): possible endocrine-like function for microbes of the gut. NPJ Biofilms Microbiomes 3:31
Esser, Lothar; Zhou, Fei; Pluchino, Kristen M et al. (2017) Structures of the Multidrug Transporter P-glycoprotein Reveal Asymmetric ATP Binding and the Mechanism of Polyspecificity. J Biol Chem 292:446-461
Azurmendi, Hugo F; Battistel, Marcos D; Zarb, Jasmin et al. (2017) The ?-reducing end in ?(2-8)-polysialic acid constitutes a unique structural motif. Glycobiology 27:900-911
Yoon, Dennis J; Chen, Kevin Y; Lopes, André M et al. (2017) Mathematical modeling of mutant transferrin-CRM107 molecular conjugates for cancer therapy. J Theor Biol 416:88-98
Xu, Xinhui; Hicks, Caitlin; Li, Yan et al. (2014) Purified cell wall from the probiotic bacterium Lactobacillus gasseri activates systemic inflammation and, at higher doses, produces lethality in a rat model. Crit Care 18:R140
Negrete, Alejandro; Pai, Amrita; Shiloach, Joseph (2014) Use of hollow fiber tangential flow filtration for the recovery and concentration of HIV virus-like particles produced in insect cells. J Virol Methods 195:240-6
Wang, Hailong; Li, Yongjiang; Truong, Lan N et al. (2014) CtIP maintains stability at common fragile sites and inverted repeats by end resection-independent endonuclease activity. Mol Cell 54:1012-21
Xiao, Su; White, Jim F; Betenbaugh, Michael J et al. (2013) Transient and stable expression of the neurotensin receptor NTS1: a comparison of the baculovirus-insect cell and the T-REx-293 expression systems. PLoS One 8:e63679

Showing the most recent 10 out of 24 publications