9602576 Scholin This objectives of this collaborative research project between Christopher A. Scholin of the Monterey Bay Aquarium Research Institute and Lesley L. Rhodes of the Cawthorn Institute in Nelson, New Zealand are (1) to determine whether the dipstick assay incorporating large-subunit ribosomal RNA (LSU rRNA)- targeted probes is a viable method for identifying and quantifying toxigenic species of Pseudo-nitzschia associated with the production of domoic acid (DA), a neurotoxic substance linked to illness of humans and wildlife (amnesic shellfish poisoning) and to economic losses, (2) to compare the performance of the dipstick assay to a microscopy-based whole cell fluorescence assay, and (3) to assess the utility of the method for routine analyses of samples collected as part of the New Zealand shellfish biotoxin monitoring program. The results will serve as a model for how one might detect other aquatic-borne toxic micro organisms in near real-time using DNA probes.

Agency
National Science Foundation (NSF)
Institute
Office of International and Integrative Activities (IIA)
Type
Standard Grant (Standard)
Application #
9602576
Program Officer
Alexander P. DeAngelis
Project Start
Project End
Budget Start
1997-04-01
Budget End
1999-03-31
Support Year
Fiscal Year
1996
Total Cost
$27,271
Indirect Cost
Name
Monterey Bay Aquarium Research Institute
Department
Type
DUNS #
City
Moss Landing
State
CA
Country
United States
Zip Code
95039