Abstract

The overall objective of this research is to determine the role of differential initiation rates on mRNAs in regulating protein synthesis in normal, virus-infected, interferon treated and transformed cells. The significance of the inhibition of the rate of translation elongation in interferon-treated cells will be assessed, especially in regard to its possible selectivity against rapidly initiating (viral) mRNAs. The mechanism by which elongation inhibition is accomplished will also be studied. The intrinsic initiation rates on various viral and host mRNAs will be measured, and the factors which determine intrinsic initiation rate will be identified. Particular attention will be paid to the role of mRNA """"""""cap"""""""" structures, and cap-specific initiation factors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020484-14
Application #
3130180
Study Section
Virology Study Section (VR)
Project Start
1978-09-30
Project End
1988-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Arts and Sciences
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Goessling, L S; Mascotti, D P; Bhattacharyya-Pakrasi, M et al. (1994) Irreversible steps in the ferritin synthesis induction pathway. J Biol Chem 269:4343-8
Daniels-McQueen, S; Goessling, L S; Thach, R E (1992) Inducible expression bovine papillomavirus shuttle vectors containing ferritin translational regulatory elements. Gene 122:271-9
Goessling, L S; Daniels-McQueen, S; Bhattacharyya-Pakrasi, M et al. (1992) Enhanced degradation of the ferritin repressor protein during induction of ferritin messenger RNA translation. Science 256:670-3
Lin, J J; Patino, M M; Gaffield, L et al. (1991) Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein. Proc Natl Acad Sci U S A 88:6068-71
Lin, J J; Daniels-McQueen, S; Gaffield, L et al. (1990) Specificity of the induction of ferritin synthesis by hemin. Biochim Biophys Acta 1050:146-50
Lin, J J; Walden, W E; Thach, R E (1990) Induction of ferritin synthesis in vitro: problems of specificity inherent in the use of iron compounds. Enzyme 44:59-67
Lin, J J; Daniels-McQueen, S; Patino, M M et al. (1990) Derepression of ferritin messenger RNA translation by hemin in vitro. Science 247:74-7
Lawson, T G; Smith, L L; Palmenberg, A C et al. (1989) Inducible expression of encephalomyocarditis virus 3C protease activity in stably transformed mouse cell lines. J Virol 63:5013-22
Lawson, T G; Lee, K A; Maimone, M M et al. (1989) Dissociation of double-stranded polynucleotide helical structures by eukaryotic initiation factors, as revealed by a novel assay. Biochemistry 28:4729-34
Lawson, T G; Cladaras, M H; Ray, B K et al. (1988) Discriminatory interaction of purified eukaryotic initiation factors 4F plus 4A with the 5' ends of reovirus messenger RNAs. J Biol Chem 263:7266-76

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