Eukaryotic cells direct proteins to various subcellular locations by exploiting physical differences between organelles. However, in specific instances during cellular morphogenesis in bacteria, there is no obvious basis for subcellular discrimination by which the cell could determine the proper final destination of a protein. The establishment of the asymmetric cell division site of Bacillus subtilis during sporulation and the subsequent targeting of proteins to the sporulation septum represent such a situation. We will study the localization of the protein SpolVFA, which localizes to the septal membrane at the onset of sporulation. First, we will identify the localization signal of SpolVFA and characterize it by mutational analysis. Next we will elucidate the mechanism by which SpolVFA is sorted by examining whether it is directly inserted at the septal membrane or is targeted to the septum by lateral diffusion after general insertion into the plasma membrane. Finally, we will identify extragenic factors that mediate the proper sorting of SpolVFA. Our findings will seek to explain what landmark subcellular features bacteria discern in order to identify target locations for sorted proteins. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM072408-01
Application #
6834712
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Flicker, Paula F
Project Start
2004-07-01
Project End
2007-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$47,296
Indirect Cost
Name
Harvard University
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
082359691
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Ramamurthi, Kumaran S; Losick, Richard (2008) ATP-driven self-assembly of a morphogenetic protein in Bacillus subtilis. Mol Cell 31:406-14
Ramamurthi, Kumaran S; Clapham, Katie Rose; Losick, Richard (2006) Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis. Mol Microbiol 62:1547-57