Abstract

The specific aims of the project are to determine the sequence and nature of microvascular events in skeletal muscle, intestinal and cerebral cortical vasculature during diabetes mellitus. In addition, a correlation of the severity of microvascular disturbances and hyperglycemia will be made.
These specific aims will be accompanied by the development of chronic and semi-chronic preparations of the small intestine for study of normal and pathological vascular behavior. The sequence of vascular changes after adult onset diabetes will be studied by measurement at two weeik intervals of microvascular dimensions and pressures, tissue PO2, number and distribution of microvessels and reactivity of microvessels to dilating agents. To correlate hyperglycemia to vascular disturbances, diet restriction of Db/Db mice will be used to produce gradations of hyperglycemia: vascular measurements will be the same as above. The overall results will be used to determine which diabetic vascular problems develop first and whether their development is a function of the severity of hyperglycemia.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Modified Research Career Development Award (K04)
Project #
5K04HL001089-04
Application #
3073627
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1982-08-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Type
Schools of Medicine
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202

  Publications

Falcone, J C; Bohlen, H G (1990) EDRF from rat intestine and skeletal muscle venules causes dilation of arterioles. Am J Physiol 258:H1515-23
Boegehold, M A; Bohlen, H G (1988) Arteriolar diameter and tissue oxygen tension during muscle contraction in hypertensive rats. Hypertension 12:184-91
Unthank, J L; Bohlen, H G (1988) Lymphatic pathways and role of valves in lymph propulsion from small intestine. Am J Physiol 254:G389-98
Unthank, J L; Bohlen, H G (1988) Intestinal microvascular growth during maturation in diabetic juvenile rats. Circ Res 63:429-36
Unthank, J L; Bohlen, H G (1987) Quantification of intestinal microvascular growth during maturation: techniques and observations. Circ Res 61:616-24
Miller, B G; Connors, B A; Bohlen, H G et al. (1987) Cell and wall morphology of intestinal arterioles from 4- to 6- and 17- to 19-week-old Wistar-Kyoto and spontaneously hypertensive rats. Hypertension 9:59-68
Lash, J M; Bohlen, H G (1987) Perivascular and tissue PO2 in contracting rat spinotrapezius muscle. Am J Physiol 252:H1192-202
Miller, B G; Evan, A P; Bohlen, H G (1987) Exposure of vascular smooth muscle cells for analysis with the scanning electron microscope. Scanning Microsc 1:1295-313
Bohlen, H G (1987) Enhanced cerebral vascular regulation occurs by age 4 to 5 weeks in spontaneously hypertensive rats. Hypertension 9:325-31
Bohlen, H G (1987) Determinants of resting and passive intestinal vascular pressures in rat and rabbit. Am J Physiol 253:G587-95

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