The objectives to this procurement are: With approvals from relevant Contractor's internal review committees, and with informed consent from donors or their next-of-kin -- to collect human tissues, cells, and fluids (i.e. lung, liver, bronchus, colon, pancreas, blood urine, pleural effusions) at the time of surgery and at autopsy from patients with medical and personal histories (epidemiological profiles), also available by informed consent. Autopsies will be both immediate (2 hrs) and intermediate (12 hrs.) and specimens are to be delivered within 2 hours of availability from the pathologist, at any time of day or night, while patient profiles (stored with coded references to protect the identify of the donors) will be """"""""securely"""""""" stored in the contractor's facility until delivered to the NIH at the request of LHC. In addition, selected specimens will be examined (""""""""characterized"""""""") for histopathological condition by light and EM microscopy, in vitro viability and the detection of various cellular components such as hormones and oncogenes by histochemistry, including immunological methods.
| Zhang, S Z; Lipsky, M M; Trump, B F et al. (1990) Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell Biol Toxicol 6:219-34 |
