A Program Project which proposes: 1) To complete the isolation, molecular characterization and synthesis of a novel pituitary and placental protein, mesotrophin, or mesodermal growth factor (MGF) present in bovine and human pituitaries and human placenta; 2) To ascertain the biology and role of MGF in function of the corpus luteum, implantation and fetal developmnt; 3) To elucidate the nature of the hypothalamic control of the secretion of MGF which we already have demonstrated to exist; 4) Complete the isolation, characterization and synthesis of inhibin of follicular fluid origin; 5) To ascertain the biology of follicular inhibin(s); 6) To elucidate the nature of follicular peptides regulating follicular sensitivity to gonadotropins; 7) To pursue physiological studies with synthetic GRF (growth hormone releasing factor or somatocrinin), in vivo and in vitro; 8) To isolate, characterize and synthesize pituitary factors (other than ACTH) involved in control of pubertal adrenal androgenesis; 9) To improve the sensitivity of the current methodology for characterization of peptides; 10) To maintain or expand the current methodology for peptide synthesis; 11) To maintain and expand the current methodology for radioimmunoassays and immunotechniques; the latter three projects as part of core supports within the Program.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD009690-16
Application #
3096640
Study Section
Population Research and Training Committee (HDPR)
Project Start
1989-03-01
Project End
1990-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
16
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Whittier Institute for Diabetes & Endoc
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Guillemin, R; Perrin, M H (2008) Neuroendocrinology: past, present and future. Ann Endocrinol (Paris) 69:160
Grimes, R W; Barber, J A; Shimasaki, S et al. (1994) Porcine ovarian granulosa cells secrete insulin-like growth factor-binding proteins-4 and -5 and express their messenger ribonucleic acids: regulation by follicle-stimulating hormone and insulin-like growth factor-1. Biol Reprod 50:695-701
Mercado, M; Shimasaki, S; Ling, N et al. (1993) Effects of estrous cycle stage and pregnancy on follistatin gene expression and immunoreactivity in rat reproductive tissues: progesterone is implicated in regulating uterine gene expression. Endocrinology 132:1774-81
Zhu, X; Ling, N; Shimasaki, S (1993) Structural characterization of the rat insulin-like growth factor binding protein-6 gene. Biochem Biophys Res Commun 191:1237-43
Gao, L; Ling, N; Shimasaki, S (1993) Structure of the rat insulin-like growth factor binding protein-4 gene. Biochem Biophys Res Commun 190:1053-9
DePaolo, L V; Mercado, M; Guo, Y et al. (1993) Increased follistatin (activin-binding protein) gene expression in rat anterior pituitary tissue after ovariectomy may be mediated by pituitary activin. Endocrinology 132:2221-8
Zhu, X; Ling, N; Shimasaki, S (1993) Cloning of the rat insulin- like growth factor binding protein-5 gene and DNA sequence analysis of its promoter region. Biochem Biophys Res Commun 190:1045-52
Miyanaga, K; Shimasaki, S (1993) Structural and functional characterization of the rat follistatin (activin-binding protein) gene promoter. Mol Cell Endocrinol 92:99-109
DePaolo, L V; Bald, L N; Fendly, B M (1992) Passive immunoneutralization with a monoclonal antibody reveals a role for endogenous activin-B in mediating FSH hypersecretion during estrus and following ovariectomy of hypophysectomized, pituitary-grafted rats. Endocrinology 130:1741-3

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