Historically, research supported by this Program Project has contributed significantly to our basic understanding of the neuroendocrinological control of reproduction and growth. Continuing with this objective, the current renewal application contains projects aimed at elucidating the complex mechanisms regulating ovarian follicle growth and differentiation. A multidisciplinary approach will be utilized in which regulation of follicular growth and differentiation will be examined at the molecular, cellular and organismic levels. Specifically, projects are proposed to: (1) isolate and identify all the polypeptide growth factors present in follicular fluid as these factors may have inherent autocrine/paracrine roles in regulating folliculogenesis; (2) explore the regulation and intragonadal action of insulin-like growth factor-binding proteins; (3) ascertain the physiological importance of follistatin in reproduction; and (4) study the regulation of follistatin at the gene level. Experiments proposed in this Program Project will be supported by four Core Units. These Core Units will be established to: (A) maintain and improve the methodology for amino acid analysis and microsequencing to characterize polypeptides, as well as maintain the peptide synthesis facility to provide synthetic peptides for all of the research projects for biological studies and raising of antibodies; (B) maintain the molecular biology facility to perform routine DNA sequencing, oligonucleotide synthesis and preparation of ribo-probes for in situ hybridization studies; (C) provide a central facility for the development and performance of radioimmunoassays and (D) provide a centralized administrative core facility to oversee the daily operation of the Program Project.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD009690-18
Application #
2196683
Study Section
Population Research Committee (HDPR)
Project Start
1989-03-01
Project End
1993-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
18
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Whittier Institute for Diabetes & Endoc
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Zhu, X; Ling, N; Shimasaki, S (1993) Cloning of the rat insulin- like growth factor binding protein-5 gene and DNA sequence analysis of its promoter region. Biochem Biophys Res Commun 190:1045-52
Miyanaga, K; Shimasaki, S (1993) Structural and functional characterization of the rat follistatin (activin-binding protein) gene promoter. Mol Cell Endocrinol 92:99-109
DePaolo, L V; Bald, L N; Fendly, B M (1992) Passive immunoneutralization with a monoclonal antibody reveals a role for endogenous activin-B in mediating FSH hypersecretion during estrus and following ovariectomy of hypophysectomized, pituitary-grafted rats. Endocrinology 130:1741-3

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