Abstract

Aim 1 is to generate and characterize 10 IgG monoclonal aCLs.
Aim 2 is to analyze monoclonal aCLs in the in vivo fetal loss model.
Aim 3 is to identify and characterize the mimetics for pathogenic aCL.
Aim 4 is to do immunological and functional studies of antibodies against mimetics in APS patients.
Aim 5 is to apply mimetics to study pathogenic aCL in APS patients and controls.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
2R01AR042506-04A1
Application #
6196877
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Serrate-Sztein, Susana
Project Start
1996-02-25
Project End
2003-07-31
Budget Start
2000-08-18
Budget End
2001-07-31
Support Year
4
Fiscal Year
2000
Total Cost
$251,685
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Chen, P P; Wu, M; Hahn, B H (2010) Some antiphospholipid antibodies bind to various serine proteases in hemostasis and tip the balance toward hypercoagulant states. Lupus 19:365-9
Yang, Yao-Hsu; Chien, Daniel; Wu, Meifang et al. (2009) Novel autoantibodies against the activated coagulation factor IX (FIXa) in the antiphospholipid syndrome that interpose the FIXa regulation by antithrombin. J Immunol 182:1674-80
Ede, Kaleo; Hwang, Kwan-Ki; Wu, Chen-Ching et al. (2009) Plasmin immunization preferentially induces potentially prothrombotic IgG anticardiolipin antibodies in MRL/MpJ mice. Arthritis Rheum 60:3108-17
Pierangeli, Silvia S; Chen, Pojen P; Raschi, Elena et al. (2008) Antiphospholipid antibodies and the antiphospholipid syndrome: pathogenic mechanisms. Semin Thromb Hemost 34:236-50
Chen, P P; Yang, C D; Ede, K et al. (2008) Some antiphospholipid antibodies bind to hemostasis and fibrinolysis proteases and promote thrombosis. Lupus 17:916-21
Lin, Wei-Shiang; Chen, Pei-Chih; Yang, Cheng-De et al. (2007) Some antiphospholipid antibodies recognize conformational epitopes shared by beta2-glycoprotein I and the homologous catalytic domains of several serine proteases. Arthritis Rheum 56:1638-47
Chen, Xiao-Xiang; Gu, Yue-Ying; Li, Shu-Jie et al. (2007) Some plasmin-induced antibodies bind to cardiolipin, display lupus anticoagulant activity and induce fetal loss in mice. J Immunol 178:5351-6
Vega-Ostertag, Mariano; Liu, Xiaowei; Kwan-Ki, Hwang et al. (2006) A human monoclonal antiprothrombin antibody is thrombogenic in vivo and upregulates expression of tissue factor and E-selectin on endothelial cells. Br J Haematol 135:214-9
Yang, Yao-Hsu; Hwang, Kwan-Ki; FitzGerald, John et al. (2006) Antibodies against the activated coagulation factor X (FXa) in the antiphospholipid syndrome that interfere with the FXa inactivation by antithrombin. J Immunol 177:8219-25
Lu, Cai-Sheng; Horizon, Arash A; Hwang, Kwan-Ki et al. (2005) Identification of polyclonal and monoclonal antibodies against tissue plasminogen activator in the antiphospholipid syndrome. Arthritis Rheum 52:4018-27

Showing the most recent 10 out of 21 publications