Over the last year, our studies have been concerned predominantly with the characterization of cytoskeletal proteins in chronic lymphocytic leukemia (CLL) lymphocytes. Previous studies from this laboratory have shown that actin is a major protein of human lymphocytes. We now report the purification to homogeneity and characterization of actin from blood lymphocytes of normal subjects and patients with CLL. The possible significance of the observed differences between the myosin Mg?++?-ATPase activation by CLL and normal lymphocyte actin has been investigated. Studies have been initiated on vimentin, the major protein of intermediate filaments, and its role in normal and CLL lymphocytes. Over the next year, we will again focus on 5' nucleotidase (5'N). Specifically, the induction of 5'N in 5'N negative CLL lymphocytes by TPA (4-beta-phorbol 12-beta-myristate 13-alpha-acetate) will be investigated. These investigations will continue our long-term documentation of similarities and differences between normal and CLL lymphocytes. (B)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA011655-21
Application #
3163512
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1975-01-01
Project End
1989-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
21
Fiscal Year
1988
Total Cost
Indirect Cost
Name
New York University
Department
Type
Schools of Medicine
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012
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