Abstract

Over the last year, our studies have been concerned predominantly with the characterization of cytoskeletal proteins in chronic lymphocytic leukemia (CLL) lymphocytes. Previous studies from this laboratory have shown that actin is a major protein of human lymphocytes. We now report the purification to homogeneity and characterization of actin from blood lymphocytes of normal subjects and patients with CLL. The possible significance of the observed differences between the myosin Mg?++?-ATPase activation by CLL and normal lymphocyte actin has been investigated. Studies have been initiated on vimentin, the major protein of intermediate filaments, and its role in normal and CLL lymphocytes. Over the next year, we will again focus on 5' nucleotidase (5'N). Specifically, the induction of 5'N in 5'N negative CLL lymphocytes by TPA (4-beta-phorbol 12-beta-myristate 13-alpha-acetate) will be investigated. These investigations will continue our long-term documentation of similarities and differences between normal and CLL lymphocytes. (B)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA011655-22
Application #
3163513
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1975-01-01
Project End
1990-09-30
Budget Start
1989-01-01
Budget End
1990-09-30
Support Year
22
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
New York University
Department
Type
Schools of Medicine
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012

  Publications

Giovanella, B C; Hinz, H R; Kozielski, A J et al. (1991) Complete growth inhibition of human cancer xenografts in nude mice by treatment with 20-(S)-camptothecin. Cancer Res 51:3052-5
Woodlock, T J; Brown, R; Mani, M et al. (1990) Decreased L system amino acid transport and decreased gamma-glutamyl transpeptidase are independent processes in human chronic lymphocytic leukemia B-lymphocytes. J Cell Physiol 145:217-21
Bodley, A; Liu, L F; Israel, M et al. (1989) DNA topoisomerase II-mediated interaction of doxorubicin and daunorubicin congeners with DNA. Cancer Res 49:5969-78
Hsiang, Y H; Liu, L F; Wall, M E et al. (1989) DNA topoisomerase I-mediated DNA cleavage and cytotoxicity of camptothecin analogues. Cancer Res 49:4385-9
Jiang, B Z; Bank, B B; Hsiang, Y H et al. (1989) Lack of drug-induced DNA cross-links in chlorambucil-resistant Chinese hamster ovary cells. Cancer Res 49:5514-7
Giovanella, B C; Stehlin, J S; Wall, M E et al. (1989) DNA topoisomerase I--targeted chemotherapy of human colon cancer in xenografts. Science 246:1046-8
Silber, R; Potmesil, M; Bank, B B (1989) Studies on drug resistance in chronic lymphocytic leukemia. Adv Enzyme Regul 29:267-76
Bank, B B; Kanganis, D; Liebes, L F et al. (1989) Chlorambucil pharmacokinetics and DNA binding in chronic lymphocytic leukemia lymphocytes. Cancer Res 49:554-9
Farber, C M; Kanganis, D N; Liebes, L F et al. (1989) Antioxidant enzymes in lymphocytes from normal subjects and patients with chronic lymphocytic leukaemia: increased glutathione peroxidase activity in CLL B lymphocytes. Br J Haematol 72:32-5
Potmesil, M; Hsiang, Y H; Liu, L F et al. (1988) Resistance of human leukemic and normal lymphocytes to drug-induced DNA cleavage and low levels of DNA topoisomerase II. Cancer Res 48:3537-43

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