Abstract

We have developed a system to simplify the study of human cell surface structures by procedures which permit study of antigens due to individual chromosomes. A human-Chinese hameter, somatic cell hybrid, which contains chromosome 11 as its only human chromosome has been produced. This hybrid, which expresses human cell surface antigens, has been employed as a model system to study these components using a combined immunological, biochemical, and genetic approach. We have developed a deletion clone panel of chromosome 11, which has enabled us to conduct a variety of regional mapping studies including development of a new method using a low-repeat human DNA sequence. The production of monoclonal antibodies has allowed us to further characterize these antigens and to identify new antigens encoded by chromosome 11. We have begun experiments which should enable us to clone the genes for two of these antigens. We have recently carried out similar studies using hybrids that retain only human chromosomes 3 and 21. Deletion panels are being developed for these chromosomes and have been used for regional mapping. Studies of this kind should make possible rapid and definitive identification of a large variety of human cell surface antigens which may influence malignant behavior of human cells. The chromosomal location and biochemical identity of such genetic determinants may help elucidate genetic relationships of antigens involved in malignant response. (CS)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA018734-11
Application #
3165038
Study Section
Immunobiology Study Section (IMB)
Project Start
1976-06-30
Project End
1990-04-30
Budget Start
1986-05-01
Budget End
1987-04-30
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Carter, R E; Cerosaletti, K M; Burkin, D J et al. (1995) The gene for the serpin thrombin inhibitor (PI7), protease nexin I, is located on human chromosome 2q33-q35 and on syntenic regions in the mouse and sheep genomes. Genomics 27:196-9
Gu, Y; Cimino, G; Alder, H et al. (1992) The (4;11)(q21;q23) chromosome translocations in acute leukemias involve the VDJ recombinase. Proc Natl Acad Sci U S A 89:10464-8
Menon, R S; Chang, Y F; Jeffers, K F et al. (1992) Regional localization of human beta-casein gene (CSN2) to 4pter-q21. Genomics 13:225-6
Gerhard, D S; Lawrence, E; Wu, J et al. (1992) Isolation of 1001 new markers from human chromosome 11, excluding the region of 11p13-p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybrids. Genomics 13:1133-42
Janson, M; Larsson, C; Werelius, B et al. (1991) Detailed physical map of human chromosomal region 11q12-13 shows high meiotic recombination rate around the MEN1 locus. Proc Natl Acad Sci U S A 88:10609-13
Miller, Y E; Drabkin, H; Jones, C et al. (1990) Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18. Genomics 8:149-54
Yunis, J J; Jones, C; Madden, M T et al. (1989) Gene order, amplification, and rearrangement of chromosome band 11q23 in hematologic malignancies. Genomics 5:84-90
Emrie, P A; Jones, C; Hofmann, T et al. (1988) The coding sequence for the human 18,000-dalton hydrophobic pulmonary surfactant protein is located on chromosome 2 and identifies a restriction fragment length polymorphism. Somat Cell Mol Genet 14:105-10
Bill, J; Palmer, E; Jones, C (1987) Molecular cloning of MER-2, a human chromosome-11-encoded red blood cell antigen, using linkage of cotransfected markers. Somat Cell Mol Genet 13:553-61
Miller, Y E; Daniels, G L; Jones, C et al. (1987) Identification of a cell-surface antigen produced by a gene on human chromosome 3 (cen-q22) and not expressed by Rhnull cells. Am J Hum Genet 41:1061-70

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