The q23 region of human chromosome 11 will be examined using an immunogenetic approach that utilizes somatic cell hybrids and human cell surface antigens expressed by these hybrids. Monoclonal antibodies recognizing these antigens allow them to be used as selective markers to manipulate the loss and retention of regions of chromosome 11. More than 16 different chromosomal subtypes of leukemia and lymphoma have a rearrangement of 11q23 with a second, but varying chromosome. A malignancy associated translocation in Ewing sarcoma involves 11q23. The most common recurrent constitutional translocation is also in this chromosomal region. Regional somatic cell mapping panels will subdivide 11q23 and pulsed field gel electrophoresis will be used to locate these breakpoints. Isolation of breakpoints and comparison of their sequences will be used to test hypotheses about mechanisms of chromosomal rearrangements. The identification of gene sequences including possible proto-oncogenes should add to the understanding of the pathogenesis of malignancy.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA018734-17
Application #
3165042
Study Section
Pathology B Study Section (PTHB)
Project Start
1988-05-01
Project End
1995-11-30
Budget Start
1992-12-01
Budget End
1993-11-30
Support Year
17
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Eleanor Roosevelt Institute for Cancer Research
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206
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