Abstract

The q23 region of human chromosome 11 will be examined using an immunogenetic approach that utilizes somatic cell hybrids and human cell surface antigens expressed by these hybrids. Monoclonal antibodies recognizing these antigens allow them to be used as selective markers to manipulate the loss and retention of regions of chromosome 11. More than 16 different chromosomal subtypes of leukemia and lymphoma have a rearrangement of 11q23 with a second, but varying chromosome. A malignancy associated translocation in Ewing sarcoma involves 11q23. The most common recurrent constitutional translocation is also in this chromosomal region. Regional somatic cell mapping panels will subdivide 11q23 and pulsed field gel electrophoresis will be used to locate these breakpoints. Isolation of breakpoints and comparison of their sequences will be used to test hypotheses about mechanisms of chromosomal rearrangements. The identification of gene sequences including possible proto-oncogenes should add to the understanding of the pathogenesis of malignancy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA018734-16
Application #
3165041
Study Section
Pathology B Study Section (PTHB)
Project Start
1988-05-01
Project End
1995-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
16
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Eleanor Roosevelt Institute for Cancer Research
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Carter, R E; Cerosaletti, K M; Burkin, D J et al. (1995) The gene for the serpin thrombin inhibitor (PI7), protease nexin I, is located on human chromosome 2q33-q35 and on syntenic regions in the mouse and sheep genomes. Genomics 27:196-9
Gu, Y; Cimino, G; Alder, H et al. (1992) The (4;11)(q21;q23) chromosome translocations in acute leukemias involve the VDJ recombinase. Proc Natl Acad Sci U S A 89:10464-8
Menon, R S; Chang, Y F; Jeffers, K F et al. (1992) Regional localization of human beta-casein gene (CSN2) to 4pter-q21. Genomics 13:225-6
Gerhard, D S; Lawrence, E; Wu, J et al. (1992) Isolation of 1001 new markers from human chromosome 11, excluding the region of 11p13-p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybrids. Genomics 13:1133-42
Janson, M; Larsson, C; Werelius, B et al. (1991) Detailed physical map of human chromosomal region 11q12-13 shows high meiotic recombination rate around the MEN1 locus. Proc Natl Acad Sci U S A 88:10609-13
Miller, Y E; Drabkin, H; Jones, C et al. (1990) Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18. Genomics 8:149-54
Yunis, J J; Jones, C; Madden, M T et al. (1989) Gene order, amplification, and rearrangement of chromosome band 11q23 in hematologic malignancies. Genomics 5:84-90
Emrie, P A; Jones, C; Hofmann, T et al. (1988) The coding sequence for the human 18,000-dalton hydrophobic pulmonary surfactant protein is located on chromosome 2 and identifies a restriction fragment length polymorphism. Somat Cell Mol Genet 14:105-10
Bill, J; Palmer, E; Jones, C (1987) Molecular cloning of MER-2, a human chromosome-11-encoded red blood cell antigen, using linkage of cotransfected markers. Somat Cell Mol Genet 13:553-61
Miller, Y E; Daniels, G L; Jones, C et al. (1987) Identification of a cell-surface antigen produced by a gene on human chromosome 3 (cen-q22) and not expressed by Rhnull cells. Am J Hum Genet 41:1061-70

Showing the most recent 10 out of 18 publications