Abstract

PI abstract): The long term objective is to understand fundamental molecular organization of the projection of the vestibular efferent neurons to the vestibular periphery. The proposal addresses 4 questions: 1) what are the topographic distributions of expressed genes and proteins for transmitter signaling in the rat and human vestibular end-organs and primary afferent ganglia; 2) what are the full length cDNA sequences encoding the mRNAs subserving the efferent/afferent interaction in the vestibular periphery; 3) what is the chromosome location and genomic sequence for each of these genes and how are they processes to yield these cDNAs; and 4) what changes occur in these genes during the aging process.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC002971-09
Application #
6634473
Study Section
Special Emphasis Panel (ZRG1-IFCN-8 (03))
Program Officer
Platt, Christopher
Project Start
1995-07-01
Project End
2005-06-30
Budget Start
2003-07-01
Budget End
2004-06-30
Support Year
9
Fiscal Year
2003
Total Cost
$351,325
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Cioffi, Joseph A; Yue, Wei Ying; Mendolia-Loffredo, Sabrina et al. (2010) MicroRNA-21 overexpression contributes to vestibular schwannoma cell proliferation and survival. Otol Neurotol 31:1455-62
Popper, Paul; Winkler, John; Erbe, Christy B et al. (2008) Distribution of two-pore-domain potassium channels in the adult rat vestibular periphery. Hear Res 246:1-8
Cioffi, Joseph A; Wackym, P Ashley; Erbe, Christy B et al. (2005) Molecular characterization of two novel splice variants of G alphai2 in the rat vestibular periphery. Brain Res Mol Brain Res 137:89-97
Roche, Joseph P; Wackym, P Ashley; Cioffi, Joseph A et al. (2005) In silico analysis of 2085 clones from a normalized rat vestibular periphery 3' cDNA library. Audiol Neurootol 10:310-22
Cristobal, Ricardo; Wackym, P Ashley; Cioffi, Joseph A et al. (2005) Assessment of differential gene expression in vestibular epithelial cell types using microarray analysis. Brain Res Mol Brain Res 133:19-36
Wackym, P Ashley; Cioffi, Joseph A; Erbe, Christy B et al. (2005) G-protein Golfalpha (GNAL) is expressed in the vestibular end organs and primary afferent neurons of Rattus norvegicus. J Vestib Res 15:11-5
Erbe, Christy B; Harris, Kevin C; Runge-Samuelson, Christina L et al. (2004) Connexin 26 and connexin 30 mutations in children with nonsyndromic hearing loss. Laryngoscope 114:607-11
Cristobal, Ricardo; Wackym, P Ashley; Cioffi, Joseph A et al. (2004) Selective acquisition of individual cell types in the vestibular periphery for molecular biology studies. Otolaryngol Head Neck Surg 131:590-5
Cioffi, Joseph A; Erbe, Christy B; Raphael, Richard et al. (2003) Expression of G-protein alpha subunit genes in the vestibular periphery of Rattus norvegicus and their chromosomal mapping. Acta Otolaryngol 123:1027-34
Halum, Stacey L; Erbe, Christy B; Friedland, David R et al. (2003) Gene discovery using a human vestibular schwannoma cDNA library constructed from a patient with neurofibromatosis type 2 (NF2). Otolaryngol Head Neck Surg 128:364-71

Showing the most recent 10 out of 14 publications