Specific aims and long-term objectives. Our research is aimed at a full understanding of carbohydrate metabolism in the liver and other animal tissues: in a parallel and complementary way, we tend to give a biochemical explanation to the related congenital disorders allowing eventually an appropriate treatment. During the next few years, our research will be more specifically oriented towards the elucidation of the control of glucokinase, a further investigation of the role of fructose 2,6-bisphosphate in biology and various aspects of glycogen metabolism. We will also try to detect inborn errors of metabolism related to each of these specific subjects. Furthermore, we will pursue an intensive investigation of the newly discovered deficiency of glycerate kinase. Disease of lysosomes and peroxisomes will also be studied, in parallel with basic research on these organelles. Methodology, Liver and muscle biopsies from patients affected by various inborn errors of metabolism (mostly glycogen storage disease, fructose intolerance, congenital lacticacidemia, galactosemia, inborn lysosomal disease) are regularly received in this laboratory from many clinical centers located mostly in Western Europe but also in South American and other countries. These biopsies will be submitted to both biochemical and ultrastructural investigations. Basic research on glucokinase and glycogen metabolism will be made with extracts and purified proteins from rat liver, muscle, pancreatic islets and yeast. The unknown roles of fructose 2,6-bisphosphate in biology will be searched for by investigations. Basic research on glucokinase and glycogen metabolism will be made with extracts and purified proteins from rat liver, muscle, pancreatic islets, and yeast. The unknown roles of fructose 2,6- bisphosphate in biology will be searched for by investigating the behaviour of yeast mutants in which the genes encoding the enzymes that form or degrade fructose 2,6-bisphosphate would have been either deleted or overexpressed. The role of peroxisomes in fatty acid oxidation will be studied in partially purified peroxisomal fractions from rat liver and the carnitine derivatives formed will be analyzed by tandem mass spectrometry.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK009235-24
Application #
3224602
Study Section
Biochemistry Study Section (BIO)
Project Start
1977-06-01
Project End
1992-07-31
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
24
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Catholic University of Louvain
Department
Type
DUNS #
City
Louvain-LA-Neuve
State
Country
Belgium
Zip Code
Vamecq, J; Draye, J P; Poupaert, J H (1990) Studies on the metabolism of glycolyl-CoA. Biochem Cell Biol 68:846-51
Vamecq, J (1990) Fluorometric assay of peroxisomal oxidases. Anal Biochem 186:340-9
Vamecq, J; Grataroli, R; Draye, J P et al. (1989) Effects of dietary corn oil and salmon oil on the oxidation of fatty acids and prostaglandin E2 in rat gastric mucosa. Prostaglandins 37:335-44
Draye, J P; Vamecq, J (1989) The gluconeogenicity of fatty acids in mammals. Trends Biochem Sci 14:478-9
Vamecq, J; Draye, J P (1989) Comparison between the formation and the oxidation of dicarboxylylcarnitine esters in rat liver and skeletal muscle: possible implications for human inborn disorders of mitochondrial beta-oxidation. J Inherit Metab Dis 12:58-63
Vamecq, J; Draye, J P (1989) Peroxisomal and mitochondrial beta-oxidation of monocarboxylyl-CoA, omega-hydroxymonocarboxylyl-CoA and dicarboxylyl-CoA esters in tissues from untreated and clofibrate-treated rats. J Biochem 106:216-22
Veitch, K; Draye, J P; Van Hoof, F et al. (1988) Effects of riboflavin deficiency and clofibrate treatment on the five acyl-CoA dehydrogenases in rat liver mitochondria. Biochem J 254:477-81
Vamecq, J; Poll-The, B T; Saudubray, J M (1988) [Biochemistry of peroxisome and peroxisomal diseases] Ann Biol Clin (Paris) 46:233-58
Draye, J P; Veitch, K; Vamecq, J et al. (1988) Comparison of the metabolism of dodecanedioic acid in vivo in control, riboflavin-deficient and clofibrate-treated rats. Eur J Biochem 178:183-9
Van Hoof, F; Vamecq, J; Draye, J P et al. (1988) The catabolism of medium- and long-chain dicarboxylic acids. Biochem Soc Trans 16:423-4

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