The purpose of this proposal is to build on our development of single molecule RNA detection in living cells in real time. 1. To extend the technology for fast 4D imaging to describe the movements of RNA transcripts in the nucleus and cytoplasm. 2. To develop a method for detecting translation of single RNA molecules. To image RNA transcription in living cells and follow the individual transcripts released using high speed microscopic 4D imaging to correlate the molecular steps of processing, export and localization with the spatial and temporal colocalization with specific proteins. This work will result in the ability to define the mechanics of RNA metabolism (nuclear export, cytoplasmic transport, localization, translation and degradation) important for the normal functioning of all eukaryotic cells within a spatial and temporal cellular context heretofore not possible using current methods. ? ?
| Lionnet, Timothee; Czaplinski, Kevin; Darzacq, Xavier et al. (2011) A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods 8:165-70 |
| Ben-Ari, Ya'ara; Brody, Yehuda; Kinor, Noa et al. (2010) The life of an mRNA in space and time. J Cell Sci 123:1761-74 |
| Hocine, Sami; Singer, Robert H; Grünwald, David (2010) RNA processing and export. Cold Spring Harb Perspect Biol 2:a000752 |
| Wang, Donghai; Pezo, Rossanna C; Corner, Georgia et al. (2010) Altered dynamics of intestinal cell maturation in Apc1638N/+ mice. Cancer Res 70:5348-57 |
| Grünwald, David; Singer, Robert H (2010) In vivo imaging of labelled endogenous ?-actin mRNA during nucleocytoplasmic transport. Nature 467:604-7 |
| Park, Hye Yoon; Buxbaum, Adina R; Singer, Robert H (2010) Single mRNA tracking in live cells. Methods Enzymol 472:387-406 |
| Maier, Sandra; Daroqui, M Cecilia; Scherer, Stefan et al. (2009) Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells. J Cell Physiol 218:638-42 |
| Larson, Daniel R; Singer, Robert H; Zenklusen, Daniel (2009) A single molecule view of gene expression. Trends Cell Biol 19:630-7 |
| Dib, Amel; Glebov, Oleg K; Shou, Yaping et al. (2009) A der(8)t(8;11) chromosome in the Karpas-620 myeloma cell line expresses only cyclin D1: yet both cyclin D1 and MYC are repositioned in close proximity to the 3'IGH enhancer. DNA Repair (Amst) 8:330-5 |
| Rodriguez, Alexis J; Czaplinski, Kevin; Condeelis, John S et al. (2008) Mechanisms and cellular roles of local protein synthesis in mammalian cells. Curr Opin Cell Biol 20:144-9 |
Showing the most recent 10 out of 19 publications
