The purpose of this proposal is to build on our development of single molecule RNA detection in living cells in real time. 1. To extend the technology for fast 4D imaging to describe the movements of RNA transcripts in the nucleus and cytoplasm. 2. To develop a method for detecting translation of single RNA molecules. To image RNA transcription in living cells and follow the individual transcripts released using high speed microscopic 4D imaging to correlate the molecular steps of processing, export and localization with the spatial and temporal colocalization with specific proteins. This work will result in the ability to define the mechanics of RNA metabolism (nuclear export, cytoplasmic transport, localization, translation and degradation) important for the normal functioning of all eukaryotic cells within a spatial and temporal cellular context heretofore not possible using current methods. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB002060-24
Application #
7228462
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Zhang, Yantian
Project Start
1984-06-01
Project End
2009-05-31
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
24
Fiscal Year
2007
Total Cost
$325,233
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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