Abstract

This research establishes the experimental and theoretical foundation for the development of potentiometric ion sensors with detection limits in the micromolar to picomolar range. The flux of analyte ions from the membrane into the sample which ordinarily perturbs the sample locally at the sensing surface will be minimized by chemical means. Relevant zero-current ion fluxes within plasticized polymeric membranes will be monitored with fluorescence imaging techniques and correlated to appropriate potential drift experiments. Detailed ion flux diffusion models will be developed and correlated to experimental findings. Diffusion coefficients within the membrane phase will be systematically altered with new polyurethane materials with immobilized ionophoric components in view of low detection limit sensing applications. Such polymers will be also used to devise multi-layered membrane phases, which will be applied to generate robust ion gradients within the membrane. Miniature, micrometer sized optical sensor based on the same ionophoric membrane components will be optimized for low detection limit applications and their performance critically compared to their potentiometric counterparts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
8R01EB002189-04
Application #
6725983
Study Section
Special Emphasis Panel (ZRG1-BMT (01))
Program Officer
Peng, Grace
Project Start
2000-09-25
Project End
2005-08-31
Budget Start
2003-09-01
Budget End
2005-08-31
Support Year
4
Fiscal Year
2003
Total Cost
$157,655
Indirect Cost

  Institution

Name
Auburn University at Auburn
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
066470972
City
Auburn University
State
AL
Country
United States
Zip Code
36849

  Publications

Valdes-Ramirez, Gabriela; Windmiller, Joshua R; Claussen, Jonathan C et al. (2012) Multiplexed and Switchable Release of Distinct Fluids from Microneedle Platforms via Conducting Polymer Nanoactuators for Potential Drug Delivery. Sens Actuators B Chem 161:
Walter, Anne; Wu, Jie; Flechsig, Gerd-Uwe et al. (2011) Redox cycling amplified electrochemical detection of DNA hybridization: application to pathogen E. coli bacterial RNA. Anal Chim Acta 689:29-33
Bakker, Eric (2010) Generalized Selectivity Description for Polymeric Ion-Selective Electrodes Based on the Phase Boundary Potential Model. J Electroanal Chem (Lausanne) 639:1-7
Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Thin layer coulometry with ionophore based ion-selective membranes. Anal Chem 82:4537-42
Pawlak, Marcin; Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Ferrocene bound poly(vinyl chloride) as ion to electron transducer in electrochemical ion sensors. Anal Chem 82:6887-94
Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Background Current Elimination in Thin Layer Ion-Selective Membrane Coulometry. Electrochem commun 12:1195-1198
Xiang, Yun; Zhang, Yuyong; Qian, Xiaoqing et al. (2010) Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy. Biosens Bioelectron 25:2539-42
Morf, W E; Pretsch, E; De Rooij, N F (2010) Theoretical Treatment and Numerical Simulation of Potential and Concentration Profiles in Extremely Thin Non-Electroneutral Membranes Used for Ion-Selective Electrodes. J Electroanal Chem (Lausanne) 642:45-56
Xiang, Yun; Zhang, Yuyong; Chang, Yue et al. (2010) Reverse-micelle synthesis of electrochemically encoded quantum dot barcodes: application to electronic coding of a cancer marker. Anal Chem 82:1138-41
Veder, Jean-Pierre; Patel, Kunal; Clarke, Graeme et al. (2010) Synchrotron radiation/Fourier transform-infrared microspectroscopy study of undesirable water inclusions in solid-contact polymeric ion-selective electrodes. Anal Chem 82:6203-7

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