The discovery that the detection limit of potentiometric sensors may be improved by 3 to 6 orders of magnitude of traditionally accepted levels have drastically changed the field of ion sensing. In this continuation proposal, new avenues will be explored to more completely assess the limits of such sensors and to design novel sensing concepts based on ion fluxes at ion-selective membranes. Since potentiometry, as one-dimensional technique, is not subject to scaling laws, the limits of potentiometric sensors will be explored in confined sample volumes. This knowledge will be used to design ultra-sensitive DNA detection devices based on metal nanoparticle probes that would be potentiometrically detected after oxidation. Potentiometry will be coupled to analyte enrichment processes, in analogy to their voltammetric counterparts for a further drastic decrease in detection limit that may surpass that of any other electrochemical method. Ion fluxes at ion-selective membranes, induced chemically and galvanostatically, will be used to design 10-fold more sensitive measuring devices than in traditional potentiometry, which will be very useful for electrolyte and drug monitoring. The perturbance of such fluxes by surface binding events will be used to explore novel approaches to biosensing at liquid-polymer interfaces.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BECM (01))
Program Officer
Korte, Brenda
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Purdue University
Schools of Arts and Sciences
West Lafayette
United States
Zip Code
Valdes-Ramirez, Gabriela; Windmiller, Joshua R; Claussen, Jonathan C et al. (2012) Multiplexed and Switchable Release of Distinct Fluids from Microneedle Platforms via Conducting Polymer Nanoactuators for Potential Drug Delivery. Sens Actuators B Chem 161:
Walter, Anne; Wu, Jie; Flechsig, Gerd-Uwe et al. (2011) Redox cycling amplified electrochemical detection of DNA hybridization: application to pathogen E. coli bacterial RNA. Anal Chim Acta 689:29-33
Chumbimuni-Torres, Karin Y; Wu, Jie; Clawson, Corbin et al. (2010) Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes. Analyst 135:1618-23
Bakker, Eric (2010) Generalized Selectivity Description for Polymeric Ion-Selective Electrodes Based on the Phase Boundary Potential Model. J Electroanal Chem (Lausanne) 639:1-7
Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Thin layer coulometry with ionophore based ion-selective membranes. Anal Chem 82:4537-42
Pawlak, Marcin; Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Ferrocene bound poly(vinyl chloride) as ion to electron transducer in electrochemical ion sensors. Anal Chem 82:6887-94
Grygolowicz-Pawlak, Ewa; Bakker, Eric (2010) Background Current Elimination in Thin Layer Ion-Selective Membrane Coulometry. Electrochem commun 12:1195-1198
Xiang, Yun; Zhang, Yuyong; Qian, Xiaoqing et al. (2010) Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy. Biosens Bioelectron 25:2539-42
Morf, W E; Pretsch, E; De Rooij, N F (2010) Theoretical Treatment and Numerical Simulation of Potential and Concentration Profiles in Extremely Thin Non-Electroneutral Membranes Used for Ion-Selective Electrodes. J Electroanal Chem (Lausanne) 642:45-56
Xiang, Yun; Zhang, Yuyong; Chang, Yue et al. (2010) Reverse-micelle synthesis of electrochemically encoded quantum dot barcodes: application to electronic coding of a cancer marker. Anal Chem 82:1138-41

Showing the most recent 10 out of 65 publications