The discovery that the detection limit of potentiometric sensors may be improved by 3 to 6 orders of magnitude of traditionally accepted levels have drastically changed the field of ion sensing. In this continuation proposal, new avenues will be explored to more completely assess the limits of such sensors and to design novel sensing concepts based on ion fluxes at ion-selective membranes. Since potentiometry, as one-dimensional technique, is not subject to scaling laws, the limits of potentiometric sensors will be explored in confined sample volumes. This knowledge will be used to design ultra-sensitive DNA detection devices based on metal nanoparticle probes that would be potentiometrically detected after oxidation. Potentiometry will be coupled to analyte enrichment processes, in analogy to their voltammetric counterparts for a further drastic decrease in detection limit that may surpass that of any other electrochemical method. Ion fluxes at ion-selective membranes, induced chemically and galvanostatically, will be used to design 10-fold more sensitive measuring devices than in traditional potentiometry, which will be very useful for electrolyte and drug monitoring. The perturbance of such fluxes by surface binding events will be used to explore novel approaches to biosensing at liquid-polymer interfaces.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
2R01EB002189-05
Application #
6868497
Study Section
Special Emphasis Panel (ZRG1-BECM (01))
Program Officer
Korte, Brenda
Project Start
2000-09-25
Project End
2009-07-31
Budget Start
2005-09-01
Budget End
2006-07-31
Support Year
5
Fiscal Year
2005
Total Cost
$302,197
Indirect Cost
Name
Purdue University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
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