We will carry out several projects: 1) the high resolution immunoelectron microscopic localization of a number of recently discovered junctional proteins at a variety of membrane - microfilament attachment sites; 2) continued search for integral membrane proteins at these sites, using a monoclonal antibody approach combined with selection by immunocytochemical localization; 3) continued studies of the immunolocalization of phosphotyrosine residues of proteins inside intact cells, using specific monoclonal antibodies generated to phosphotyrosine; 4) the extension of our immunoelectron microscopic studies of the intracellular pathways of serum protein secretion in cultured hepatocytes; and 5) the exploration of the molecular basis for the association which we have demonstrated between the Golgi apparatus and the microtubule organizing center in cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM015971-19
Application #
3268870
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1978-05-01
Project End
1991-04-30
Budget Start
1986-05-01
Budget End
1987-04-30
Support Year
19
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093