Abstract

We will theoretically and experimentally analyze the vibrational modes of double helical DNA. We will use this fundamental knowledge to explore significant phenomena involving atomic motions of the helix. Specific phenomena are melting of the helix, enzyme driven melting, enzyme propulsion and conformation change. We will analyze the relationship between dichroic angle and conformation. We will measure f.i.r. spectra and refine force constants for DNA helix and many of its constituent parts. We plan a double resonance experiment to measure mode interactions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM024443-07
Application #
3272310
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1980-01-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Prabhu, V V; Young, L; Prohofsky, E W (1991) Observation of preferred direction melting in a lattice model of poly(dA-dC).poly(dG-dT). Biopolymers 31:355-8
Chen, Y Z; Feng, Y; Prohofsky, E W (1991) Premelting thermal fluctuational base pair opening probability of poly(dA).poly(dT) as predicted by the modified self-consistent phonon theory. Biopolymers 31:139-48
Beger, R; Feng, Y; Prohofsky, E W (1990) Vibrational fluctuations of hydrogen bonds in a DNA double helix with alternating TA type inserts. Biophys J 58:437-45
Feng, Y; Prohofsky, E W (1990) Vibrational fluctuations of hydrogen bonds in a DNA double helix with nonuniform base pairs. Biophys J 57:547-53
Schroll, W K; Prabhu, V V; Prohofsky, E W et al. (1989) Phonon interpretation of inelastic neutron scattering in DNA crystals. Biopolymers 28:1189-93
Girirajan, K S; Young, L; Prohofsky, E W (1989) Vibrational free energy, entropy, and temperature factors of DNA calculated by a helix lattice approach. Biopolymers 28:1841-60
Hua, X M; Prohofsky, E W (1988) Normal-mode calculation for methylated Z-DNA poly(dG-m5dC).(dG-m5dC). Biopolymers 27:645-55
Kim, Y; Prohofsky, E W (1986) Predicted role of particular phonons in the strand separation melting of the DNA double helix. J Biomol Struct Dyn 4:437-42
Devi Prasad, K V; Prohofsky, E W (1985) Conformational changes in DNA and soft modes. J Biomol Struct Dyn 3:551-8