Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028988-14
Application #
2175351
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1981-04-01
Project End
1997-03-31
Budget Start
1995-04-01
Budget End
1997-03-31
Support Year
14
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Barthel, T K; Walker, G C (1999) Inferences concerning the ATPase properties of DnaK and other HSP70s are affected by the ADP kinase activity of copurifying nucleoside-diphosphate kinase. J Biol Chem 274:36670-8
Herman, C; Thevenet, D; Bouloc, P et al. (1998) Degradation of carboxy-terminal-tagged cytoplasmic proteins by the Escherichia coli protease HflB (FtsH). Genes Dev 12:1348-55
Zhang, J; Walker, G C (1998) Interactions of peptides with DnaK and C-terminal DnaK fragments studied using fluorescent and radioactive peptides. Arch Biochem Biophys 356:177-86
Zhang, J; Walker, G C (1996) Identification of elements of the peptide binding site of DnaK by peptide cross-linking. J Biol Chem 271:19668-74
Zhang, J; Lee, M H; Walker, G C (1995) P-azidoiodoacetanilide, a new short photocrosslinker that has greater cysteine specificity than p-azidophenacyl bromide and p-azidobromoacetanilide. Biochem Biophys Res Commun 217:1177-84
McCarty, J S; Walker, G C (1994) DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation. J Bacteriol 176:764-80
Donnelly, C E; Murli, S; Walker, G C (1994) The groE gene products of Escherichia coli are dispensable for mucA+B(+)-dependent UV mutagenesis. Mutat Res 309:225-33
Bukau, B; Reilly, P; McCarty, J et al. (1993) Immunogold localization of the DnaK heat shock protein in Escherichia coli cells. J Gen Microbiol 139:95-9
Donnelly, C E; Walker, G C (1992) Coexpression of UmuD' with UmuC suppresses the UV mutagenesis deficiency of groE mutants. J Bacteriol 174:3133-9
McCarty, J S; Walker, G C (1991) DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity. Proc Natl Acad Sci U S A 88:9513-7

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