Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM038714-10
Application #
2179479
Study Section
Special Emphasis Panel (ZRG3-BBCA (01))
Project Start
1987-07-01
Project End
2000-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
Schools of Arts and Sciences
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Thompson, T M; Mark, B L; Gray, C W et al. (1998) Circular dichroism and electron microscopy of a core Y61F mutant of the F1 gene 5 single-stranded DNA-binding protein and theoretical analysis of CD spectra of four Tyr --> Phe substitutions. Biochemistry 37:7463-77
Su, S; Gao, Y G; Zhang, H et al. (1997) Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography. Protein Sci 6:771-80
Zhang, H; Skinner, M M; Sandberg, W S et al. (1996) Context dependence of mutational effects in a protein: the crystal structures of the V35I, I47V and V35I/I47V gene V protein core mutants. J Mol Biol 259:148-59
Terwilliger, T C (1996) Gene V protein dimerization and cooperativity of binding of poly(dA). Biochemistry 35:16652-64
Mark, B L; Terwilliger, T C; Vaughan, M R et al. (1995) Circular dichroism spectroscopy of three tyrosine-to-phenylalanine substitutions of fd gene 5 protein. Biochemistry 34:12854-65
Liang, H; Sandberg, W S; Terwilliger, T C (1993) Genetic fusion of subunits of a dimeric protein substantially enhances its stability and rate of folding. Proc Natl Acad Sci U S A 90:7010-4
Liang, H; Terwilliger, T C (1991) Reversible denaturation of the gene V protein of bacteriophage f1. Biochemistry 30:2772-82
Zabin, H B; Horvath, M P; Terwilliger, T C (1991) Approaches to predicting effects of single amino acid substitutions on the function of a protein. Biochemistry 30:6230-40
Sandberg, W S; Terwilliger, T C (1989) Influence of interior packing and hydrophobicity on the stability of a protein. Science 245:54-7
Terwilliger, T C (1988) Simple and highly efficient site-specific mutagenesis, by ligation of an oligodeoxyribonucleotide into gapped heteroduplex DNA in which the template strand contains deoxyuridine. Gene 69:317-24

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