Abstract

Dr. Brennwald's aims are: to (1) localize and molecularly dissect the Sec9 interacting proteins Sni1/2; (2) to characterize the function of Rho3 as a positive regulator of exocytosis by testing the effects of gain and loss of function mutations; and (3) to identify downstream effectors of Rho 3.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM054712-03
Application #
6181118
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Shapiro, Bert I
Project Start
1998-05-01
Project End
2003-04-30
Budget Start
2000-05-01
Budget End
2001-04-30
Support Year
3
Fiscal Year
2000
Total Cost
$246,727
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Jeschke, Grace R; Lou, Hua Jane; Weise, Keith et al. (2018) Substrate priming enhances phosphorylation by the budding yeast kinases Kin1 and Kin2. J Biol Chem 293:18353-18364
Rossi, Guendalina; Watson, Kelly; Kennedy, Wade et al. (2018) The tomosyn homologue, Sro7, is a direct effector of the Rab GTPase, Sec4, in post-Golgi vesicle tethering. Mol Biol Cell 29:1476-1486
Watson, Kelly; Rossi, Guendalina; Temple, Brenda et al. (2015) Structural basis for recognition of the Sec4 Rab GTPase by its effector, the Lgl/tomosyn homologue, Sro7. Mol Biol Cell 26:3289-300
Rossi, Guendalina; Watson, Kelly; Demonch, Mallory et al. (2015) In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7. J Biol Chem 290:612-24
Watson, Leah J; Rossi, Guendalina; Brennwald, Patrick (2014) Quantitative analysis of membrane trafficking in regulation of Cdc42 polarity. Traffic 15:1330-43
Brennwald, Patrick (2013) Membrane traffic: the exocyst meets the cell cycle. Curr Biol 23:R838-40
Forsmark, Annabelle; Rossi, Guendalina; Wadskog, Ingrid et al. (2011) Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion. Traffic 12:740-53
Rossi, Guendalina; Brennwald, Patrick (2011) Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis. Mol Biol Cell 22:842-57
Boulter, Etienne; Garcia-Mata, Rafael; Guilluy, Christophe et al. (2010) Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. Nat Cell Biol 12:477-83
Wu, Hao; Turner, Courtney; Gardner, Jimmy et al. (2010) The Exo70 subunit of the exocyst is an effector for both Cdc42 and Rho3 function in polarized exocytosis. Mol Biol Cell 21:430-42

Showing the most recent 10 out of 28 publications