Abstract

Based on their previous studies and preliminary data, the investigators hypothesize that during the initial stage of stromal differentiation, alphaSMA expression is regulated by the interaction between stromal cell integrins with the secreted extracellular matrix proteins laminin and fibronectin, and that during the second phase, a trophoblast factor, whose signal is mediated by cAMP, induces IGFBP-1 expression in decidualizing stromal fibroblasts, which in turn can downregulate alphaSMA. To investigate these hypotheses, they propose to characterize the mechanisms by which the integrin/ECM interactions could potentially regulate alphaSMA expression in isolated baboon stromal fibroblasts (Aim 1), identify the transcription factors that regulate IGFBP-1 gene expression in baboon decidual cells (Aim 2), and develop an in vitro implantation model to define the role of the baboon trophoblast in regulating the decidualization process (Aim 3).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD036759-03
Application #
6363418
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Yoshinaga, Koji
Project Start
1999-03-01
Project End
2004-02-29
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
3
Fiscal Year
2001
Total Cost
$279,436
Indirect Cost

  Institution

Name
University of Illinois at Chicago
Department
Obstetrics & Gynecology
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Banerjee, Prajna; Fazleabas, Asgerally T (2010) Endometrial responses to embryonic signals in the primate. Int J Dev Biol 54:295-302
Ihnatovych, Ivanna; Hu, WenYang; Martin, Jody L et al. (2007) Increased phosphorylation of myosin light chain prevents in vitro decidualization. Endocrinology 148:3176-84
Sherwin, J R A; Sharkey, A M; Cameo, P et al. (2007) Identification of novel genes regulated by chorionic gonadotropin in baboon endometrium during the window of implantation. Endocrinology 148:618-26
Strakova, Zuzana; Mavrogianis, Patricia; Meng, Xuemei et al. (2005) In vivo infusion of interleukin-1beta and chorionic gonadotropin induces endometrial changes that mimic early pregnancy events in the baboon. Endocrinology 146:4097-104
Jasinska, A; Han, V; Fazleabas, A T et al. (2004) Induction of insulin-like growth factor binding protein-1 expression in baboon endometrial stromal cells by cells of trophoblast origin. J Soc Gynecol Investig 11:399-405
Fazleabas, A T; Kim, J J; Strakova, Z (2004) Implantation: embryonic signals and the modulation of the uterine environment--a review. Placenta 25 Suppl A:S26-31
Kim, J J; Fazleabas, Asgerally T (2004) Uterine receptivity and implantation: the regulation and action of insulin-like growth factor binding protein-1 (IGFBP-1), HOXA10 and forkhead transcription factor-1 (FOXO-1) in the baboon endometrium. Reprod Biol Endocrinol 2:34
Strakova, Zuzana; Szmidt, Maciej; Srisuparp, Santha et al. (2003) Inhibition of matrix metalloproteinases prevents the synthesis of insulin-like growth factor binding protein-1 during decidualization in the baboon. Endocrinology 144:5339-46
Kim, J Julie; Taylor, Hugh S; Akbas, G Eda et al. (2003) Regulation of insulin-like growth factor binding protein-1 promoter activity by FKHR and HOXA10 in primate endometrial cells. Biol Reprod 68:24-30
Morales, Pedro J; Pace, Judith L; Platt, Jeralyn Sue et al. (2003) Placental cell expression of HLA-G2 isoforms is limited to the invasive trophoblast phenotype. J Immunol 171:6215-24

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