Abstract

The objective is to improve understanding of the endocrine and para-endocrine functions of the lungs by defining structure-function correlations of the cells that make up the alveolar-capillary unit with particular focus on the role of endothelial cells. We plan to use new techniques of electron microscopy and cell culture in efforts to define the topography of angiotensin converting enzyme at the ultrastructural level, to obtain pure capillary endothelium in culture and to examine for postulated metabolic activities of pulmonary endothelium such as the dehydration of bicarbonate and the inactivation (in terms of platelet aggregation) of adenosine-5' -diphosphate.
This aim will include studies to define the cellular and subcellular distribution of carbonic anhydrase, ADPase, aminopeptidase A and kinin converting enzyme.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL021568-09
Application #
3336574
Study Section
Pathology A Study Section (PTHA)
Project Start
1977-02-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Miami School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
Miami
State
FL
Country
United States
Zip Code
33101

  Publications

Meng, Y Y; Trachtenburg, J; Ryan, U S et al. (1995) Potentiation of endogenous nitric oxide with superoxide dismutase inhibits platelet-mediated thrombosis in injured and stenotic arteries. J Am Coll Cardiol 25:269-75
Paler-Martinez, A; Panus, P C; Chumley, P H et al. (1994) Endogenous xanthine oxidase does not significantly contribute to vascular endothelial production of reactive oxygen species. Arch Biochem Biophys 311:79-85
Trachtenberg, J D; Sun, S; Choi, E T et al. (1993) Effect of endothelin-1 infusion on the development of intimal hyperplasia after balloon catheter injury. J Cardiovasc Pharmacol 22 Suppl 8:S355-9
Ryan, U S; Zhong, R; Hayes, B A et al. (1993) Regulation of endothelin-1 expression in normal and transfected endothelial cells. J Cardiovasc Pharmacol 22 Suppl 8:S38-41
Garrick, R A; Ryan, U S; Bower, V et al. (1993) The diffusional transport of water and small solutes in isolated endothelial cells and erythrocytes. Biochim Biophys Acta 1148:108-16
Yabkowitz, R; Mansfield, P J; Ryan, U S et al. (1993) Thrombospondin mediates migration and potentiates platelet-derived growth factor-dependent migration of calf pulmonary artery smooth muscle cells. J Cell Physiol 157:24-32
Glassberg, M K; Nolop, K B; Jackowski, J T et al. (1992) Microvascular and macrovascular endothelial cells produce different constrictor substances. J Appl Physiol 72:1681-6
Visner, G A; Chesrown, S E; Monnier, J et al. (1992) Regulation of manganese superoxide dismutase: IL-1 and TNF induction in pulmonary artery and microvascular endothelial cells. Biochem Biophys Res Commun 188:453-62
Stevens, S L; Hilgarth, K; Ryan, U S et al. (1992) The synergistic effect of hypercholesterolemia and mechanical injury on intimal hyperplasia. Ann Vasc Surg 6:55-61
Marczin, N; Ryan, U S; Catravas, J D (1992) Endothelial cGMP does not regulate basal release of endothelium-derived relaxing factor in culture. Am J Physiol 263:L113-21

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