The goal of this proposal is to understand the structural features of apoB that are necessary and sufficient for the assembly of apoB-containing lipoproteins. The proposed research is driven by two hypotheses: 1) The N-terminal 20-27 percent of apoB forms an intermediate structure, homologous to lipovitellin, that contains a lipid pocket required to initiate the assembly of apoB-containing lipoprotein particles. 2) The domain of apoB homologous to lipovitellin is missing a portion of the full set of lipovitellin-like structural elements necessary for the lipid pocket formation; these missing structural elements are present in microsomal triglyceride transfer protein (MTP). The binding of MTP to the nascent apoB is necessary for structural complementation to form a complete and functional lipid pocket. These hypotheses will be tested by the three specific aims: 1) To determine the regions of the N-terminal domain of apoB that are required for the assembly of apoB-containing lipoproteins.
This aim will be accomplished by construction and expression of a library of C-terminally truncated forms of apoB and analysis of particles secreted by transfected mammalian cells in culture. We propose to map the effects on apoB expression and assembly into lipoprotein particles of sequential inclusion of the N-terminal regions of apoB, focussing on those domains structurally homologous to the key structural domains of the lipovitellin lipid pocket. 2) To develop an all atom molecular model of the lipid pocket for apoB-containing lipoprotein particle assembly in the absence and in combination with MTP. To accomplish this, lipovitellin structural homologue domains of apoB and MTP, identified by us previously using sequence similarity analysis and determination of domains rich in amphipathic motifs, will be mapped onto the structural coordinates of the lamprey lipovitellin PDB file. 3) To understand the contribution of small motifs of apoB and MTP in the assembly of apoB-containing lipoprotein particles.
This aim will be accomplished by testing the effects of site-directed mutagenesis of the N-terminal domain of apoB and the hypothesized complementary domains of MTP on the assembly of apoB-containing particles in mammalian cells in culture. The results of specific aims 1 and 2 will determine the specific sites and types of mutations selected.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL063417-02
Application #
6184831
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1999-07-01
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
2
Fiscal Year
2000
Total Cost
$255,411
Indirect Cost
Name
University of Alabama Birmingham
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294