This application proposes to examine the role that three glial cell populations play in the development of the corpus callosum. One of these populations form the glial sling, a group of cells that underlie the corpus callosum forming a """"""""bridge"""""""" between the two hemispheres. Two newly discovered populations are the glial wedge, a group of cells near the midline that are proposed to deflect cortical axons medially and the indusium griseum, a group of glial cells located dorsal to the callosum. The three specific aims proposed in this new application are as follows: (1) characterize the role Emx1 plays in regulating the expression of guidance molecules derived from the glial sling; (2) determine whether two other midline glial populations are also important for midline guidance of cortical axons; and (3) examine the development of midline glial cells in mouse mutants exhibiting an acallosal phenotype i.e. the netrin-1 and DCC mutant mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
3R01NS037792-03S1
Application #
6562864
Study Section
Special Emphasis Panel (ZRG1 (01))
Program Officer
Finkelstein, Robert
Project Start
1999-07-01
Project End
2003-04-30
Budget Start
2001-05-01
Budget End
2002-04-30
Support Year
3
Fiscal Year
2002
Total Cost
$35,000
Indirect Cost
Name
University of Maryland Baltimore
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Shu, Tianzhi; Li, Ying; Keller, Asaf et al. (2003) The glial sling is a migratory population of developing neurons. Development 130:2929-37
Shu, Tianzhi; Puche, Adam C; Richards, Linda J (2003) Development of midline glial populations at the corticoseptal boundary. J Neurobiol 57:81-94
Shu, Tianzhi; Sundaresan, Vasi; McCarthy, Margaret M et al. (2003) Slit2 guides both precrossing and postcrossing callosal axons at the midline in vivo. J Neurosci 23:8176-84
Shu, Tianzhi; Butz, Kenneth G; Plachez, Celine et al. (2003) Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice. J Neurosci 23:203-12
Rash, B G; Richards, L J (2001) A role for cingulate pioneering axons in the development of the corpus callosum. J Comp Neurol 434:147-57
Shu, T; Richards, L J (2001) Cortical axon guidance by the glial wedge during the development of the corpus callosum. J Neurosci 21:2749-58
Shu, T; Shen, W B; Richards, L J (2001) Development of the perforating pathway: an ipsilaterally projecting pathway between the medial septum/diagonal band of Broca and the cingulate cortex that intersects the corpus callosum. J Comp Neurol 436:411-22
Shu, T; Valentino, K M; Seaman, C et al. (2000) Expression of the netrin-1 receptor, deleted in colorectal cancer (DCC), is largely confined to projecting neurons in the developing forebrain. J Comp Neurol 416:201-12