Abstract

A variety of biochemical and genetic techniques will be used to study the replication of retroviruses and some of the genes implicated in retroviral oncogenesis. In the studies on viral replication, emphasis will be placed upon receptors that mediate virus entry, the mechanism of proviral integration, synthesis of viral proteins via ribosomal frameshifting, and assembly of viral envelopes. Two classes of proto-oncogenes, the src and Wnt gene families, will be examined in efforts to understand the role of their protein products in signalling in individual cells, development in whole organisms, transformation of cultured cells, and multi-step oncogenesis in transgenic animals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R35)
Project #
5R35CA039832-09
Application #
3479165
Study Section
Special Emphasis Panel (SRC (88))
Project Start
1985-08-01
Project End
1995-02-28
Budget Start
1993-08-01
Budget End
1994-07-31
Support Year
9
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Bates, P; Rong, L; Varmus, H E et al. (1998) Genetic mapping of the cloned subgroup A avian sarcoma and leukosis virus receptor gene to the TVA locus. J Virol 72:2505-8
Lee, S I; Umen, J G; Varmus, H E (1995) A genetic screen identifies cellular factors involved in retroviral -1 frameshifting. Proc Natl Acad Sci U S A 92:6587-91
Taylor, I C; Roy, S; Yaswen, P et al. (1995) Mouse mammary tumors express elevated levels of RNA encoding the murine homology of SKY, a putative receptor tyrosine kinase. J Biol Chem 270:6872-80
Verderame, M F; Varmus, H E (1994) Highly conserved amino acids in the SH2 and catalytic domains of v-src are altered in naturally occurring, transformation-defective alleles. Oncogene 9:175-82
Bates, P; Young, J A; Varmus, H E (1993) A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor. Cell 74:1043-51
Parkin, N T; Kitajewski, J; Varmus, H E (1993) Activity of Wnt-1 as a transmembrane protein. Genes Dev 7:2181-93
Mason, J O; Kitajewski, J; Varmus, H E (1992) Mutational analysis of mouse Wnt-1 identifies two temperature-sensitive alleles and attributes of Wnt-1 protein essential for transformation of a mammary cell line. Mol Biol Cell 3:521-33
Jue, S F; Bradley, R S; Rudnicki, J A et al. (1992) The mouse Wnt-1 gene can act via a paracrine mechanism in transformation of mammary epithelial cells. Mol Cell Biol 12:321-8
Kaplan, J M; Varmus, H E; Bishop, J M (1990) The src protein contains multiple domains for specific attachment to membranes. Mol Cell Biol 10:1000-9
Kamb, A; Weir, M; Rudy, B et al. (1989) Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification. Proc Natl Acad Sci U S A 86:4372-6

Showing the most recent 10 out of 12 publications