A new analytical assay was developed, using gas liquid chromatography with nitrogen phosphorus detection, for the cholinergic agonist arecoline (1, 2, 5, 6-tetrahydro-lmethyl-3-pyridinecarboxylic acid methyl ester). The assay was applied to plasma and tissue samples of animals and to plasma samples of humans. A model was developed for interpretation of the role of drug binding to plasma proteins in determining brain uptake of highly-bound drugs. The model took into account cerebral blood flow and the kinetics of protein binding.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000128-05
Application #
4687903
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code