We are studying a series of patients with a preliminary diagnosis of large granular lymphoproliferative disorder (LGLPD) using flow cytometric immunophenotyping as well as PCR and restriction enzyme digests for clonal T-cell populations. We have found that presence of cytopenias and observation of large granular lymphocytes in the peripheral blood are not sensitive indicators of LGLPD. We have detected B-cell neoplasms and non-cytotoxic T-cell proliferations (non-clonal) and malignancies in patients with cytopenias and peripheral blood large granular lymphocytes. Flow cytometric analysis is more sensitive than PCR (reported 60-70% sensitivity) in detecting T-cell malignancies in LGLPD. Restriction enzyme digests are being performed and sensitivity of detection will be compared. We are using multiparametric approaches to improve the sensitivity of detection of monoclonal B-cell populations. By targeting abnormal patterns of antigen expression (eg CD10, CD5, CD23, FMC7) by B-cells in light chain detection, we are attempting to achieve a pure tumor gate among admixed polyclonal B-cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC009372-05
Application #
2464521
Study Section
Special Emphasis Panel (LP)
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1996
Total Cost
Indirect Cost
Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
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