""""""""Flow Cytometric immunophenotyping is a sensitive technique for analysis of benign and malignant tumors. We are studying the refinement of this technique and its application to diagnosis and measurement of prognostic markers in different systems. We are studying the flow cytometric immunophenotype of CLL and correlating the expression of specific antigens with morphology, cytogenetics and clinical course. Data from this study may provide prognostic markers for this disease. We have continued our studies of myelodysplastic syndrome (MDS) and have defined diagnostic indicators of abnormal maturation in myeloid, megakaryocytic and erythroid lineages. We have shown that flow cytometric immunophenotyping is more sensitive than morphology in MDS. We are currently correlating cytogenetic abnormalities with immunophenotype in this disease. This information is being used to improve our ability to diagnose early myelodysplasia and differentiate it from other conditions with cytopenias. We have refined methods for flow cytometric monitoring of patient specimens for apoptosis induced by chemotherapy and have been able to detect apoptosis in specific cell populations. This has allowed us to demonstrate different sensitivities of specific cell lineages to chemotherapeutic agents. We have initiated a study of clonal cytotoxic T-cell populations that arise in patients with B-cell neoplasia to determine prognostic importance as well as resulting difficulties in minimal residual disease detection. The laboratory has an ongoing interest in detection of minimal and residual lymphoma. We are using multiparametric approaches to improve the sensitivity of detection of monoclonal B-cell populations. By targeting abnormal patterns of antigen expression (eg CD10, CD5, CD23, FMC7, or abnormal intensity of antigen expression) by neoplastic B-cells in light chain detection, we are attempting to detect very small numbers of neoplastic B-cells among admixed polyclonal B-cells.""""""""

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC009372-07
Application #
6123724
Study Section
Special Emphasis Panel (LP)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1998
Total Cost
Indirect Cost
Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Stetler-Stevenson, M; Yuan, C M (2009) Myelodysplastic syndromes: the role of flow cytometry in diagnosis and prognosis. Int J Lab Hematol 31:479-83
O'Mahony, Deirdre; Morris, John C; Stetler-Stevenson, Maryalice et al. (2009) EBV-related lymphoproliferative disease complicating therapy with the anti-CD2 monoclonal antibody, siplizumab, in patients with T-cell malignancies. Clin Cancer Res 15:2514-22
Arons, Evgeny; Sorbara, Lynn; Raffeld, Mark et al. (2006) Characterization of T-cell repertoire in hairy cell leukemia patients before and after recombinant immunotoxin BL22 therapy. Cancer Immunol Immunother 55:1100-10
Schinstine, Malcolm; Filie, Armando C; Wilson, Wyndham et al. (2006) Detection of malignant hematopoietic cells in cerebral spinal fluid previously diagnosed as atypical or suspicious. Cancer 108:157-62
Arons, Evgeny; Margulies, Inger; Sorbara, Lynn et al. (2006) Minimal residual disease in hairy cell leukemia patients assessed by clone-specific polymerase chain reaction. Clin Cancer Res 12:2804-11
Fogarty, Patrick F; Stetler-Stevenson, Maryalice; Pereira, Aloysius et al. (2005) Large granular lymphocytic proliferation-associated cyclic thrombocytopenia. Am J Hematol 79:334-6
Lamb Jr, Lawrence S; Neuberg, Ronnie; Welsh, Jeff et al. (2005) T-cell lymphoblastic leukemia/lymphoma syndrome with eosinophilia and acute myeloid leukemia. Cytometry B Clin Cytom 65:37-41
Ahmad, Ejaz; Kingma, Douglas W; Jaffe, Elaine S et al. (2005) Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow. Cytometry B Clin Cytom 67:6-12
Hegde, Upendra; Filie, Armando; Little, Richard F et al. (2005) High incidence of occult leptomeningeal disease detected by flow cytometry in newly diagnosed aggressive B-cell lymphomas at risk for central nervous system involvement: the role of flow cytometry versus cytology. Blood 105:496-502
Marti, Gerald E; Vogt Jr, Robert F; Stetler-Stevenson, Maryalice (2003) Clinical quantitative flow cytometry: ""Identifying the optimal methods for clinical quantitative flow cytometry"". Cytometry B Clin Cytom 55:59

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