Abstract

The purpose of the project is to find out how microbial molecules, such as bacterial lipopolysaccharides, induce the accumulation of triglycerides (i.e., fat droplets) in macrophages. This process is important for the pathogenesis of the foamy macrophages that are found at sites of infection (especially within granulomas) and in arterial walls (where macrophages contribute substantially to atherosclerosis). This project has matured nicely during the past year and a manuscript should be submitted for publication soon.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001138-02
Application #
8556043
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2012
Total Cost
$337,751
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Lu, Mingfang; Munford, Robert (2016) LPS stimulates IgM production in vivo without help from non-B cells. Innate Immun 22:307-15
Munford, Robert S (2016) Endotoxemia-menace, marker, or mistake? J Leukoc Biol 100:687-698
Bachovchin, Daniel A; Koblan, Luke W; Wu, Wengen et al. (2014) A high-throughput, multiplexed assay for superfamily-wide profiling of enzyme activity. Nat Chem Biol 10:656-63
Lu, Mingfang; Kho, Terry; Munford, Robert S (2014) Prolonged triglyceride storage in macrophages: pHo trumps pO2 and TLR4. J Immunol 193:1392-7
Huang, Ying-ling; Morales-Rosado, Joel; Ray, Jessica et al. (2014) Toll-like receptor agonists promote prolonged triglyceride storage in macrophages. J Biol Chem 289:3001-12
Lu, Mingfang; Varley, Alan W; Munford, Robert S (2013) Persistently active microbial molecules prolong innate immune tolerance in vivo. PLoS Pathog 9:e1003339
Shao, Baomei; Munford, Robert S; Kitchens, Richard et al. (2012) Hepatic uptake and deacylation of the LPS in bloodborne LPS-lipoprotein complexes. Innate Immun 18:825-33
Suffredini, Anthony F; Munford, Robert S (2011) Novel therapies for septic shock over the past 4 decades. JAMA 306:194-9
Shao, Baomei; Kitchens, Richard L; Munford, Robert S et al. (2011) Prolonged hepatomegaly in mice that cannot inactivate bacterial endotoxin. Hepatology 54:1051-62
Lu, Mingfang; Munford, Robert S (2011) The transport and inactivation kinetics of bacterial lipopolysaccharide influence its immunological potency in vivo. J Immunol 187:3314-20