This project was recently initiated. Progress is primarily at the level of developing the necessary infrastructure to accomplish the project goals. As osteosarcoma is a rare cancer, obtaining suitable biospecimens is a major obstacle to studying this disease. To overcome this problem it has been necessary to reach outside of the intramural community. A collaboration to obtain specimens has been established with the Childrens Oncology Group (COG), the primary national cooperative clinical trials group for pediatric cancer, to sequence specimens collected under COG trial. As osteosarcoma had been selected by the TARGET project (NCIs Therapeutically Applicable Research to Generate Effective Treatments,, the TARGET infrastructure has been used to facilitate the COG collaboration. This collaboration will provide samples for analysis. An additional aspect of the early phase of this project has been the development of the necessary sequencing infrastructure. Suitable sequencing and computational equipment has been installed to support this project, and the bioinformatics infrastructure for data analysis has also been developed. Data generation has begun on a panel of osteosarcoma cell lines. This will be highly useful for the long term goals of the project since well characterized cell lines can be used for functional investigation of the biologically important results obtained in the course of this research. We have now initiated the analysis of clinical biospecimens collected through the TARGET program.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
National Cancer Institute Division of Basic Sciences
Zip Code
Nakka, Manjula; Allen-Rhoades, Wendy; Li, Yiting et al. (2017) Biomarker significance of plasma and tumor miR-21, miR-221, and miR-106a in osteosarcoma. Oncotarget 8:96738-96752
Gindin, Yevgeniy; Jiang, Yuan; Francis, Princy et al. (2015) miR-23a impairs bone differentiation in osteosarcoma via down-regulation of GJA1. Front Genet 6:233
Mirabello, Lisa; Koster, Roelof; Moriarity, Branden S et al. (2015) A Genome-Wide Scan Identifies Variants in NFIB Associated with Metastasis in Patients with Osteosarcoma. Cancer Discov 5:920-31
Khanna, Chand; Fan, Timothy M; Gorlick, Richard et al. (2014) Toward a drug development path that targets metastatic progression in osteosarcoma. Clin Cancer Res 20:4200-9
Savage, Sharon A; Mirabello, Lisa; Wang, Zhaoming et al. (2013) Genome-wide association study identifies two susceptibility loci for osteosarcoma. Nat Genet 45:799-803