The flagellar regulon presents a unique opportunity to study interoperon and global transcriptional regulation. Within the 14 operons in this regulon, there exists a variety of novel mechanisms that ensure the coordination of temporal expression and of transcription with the assembly of the flagellar structure itself. Additionally, it has been shown that one of the gene products of the """"""""master"""""""" regulatory operon, FlhD, is a global regulator. This proposal will study how the FlhD/C heterotetramer regulates the flagellar regulon and how FlhD without FlhC regulates other operons. FlhD/C heterotetramer has a different DNA binding specificity than does FlhD by itself. The investigator will determine how the changes in the composition of these complexes alter DNA binding specificity. He will continue comprehensive structure/function analyses of FlhD and complexes containing FlhD. He will use allele-specific suppression analysis and X-ray crystallography to determine how FlhD interacts with FlhC and other possible complex partners. He will also characterize other nonflagellar DNA targets of FlhD and determine how the FlhD- containing complexes interact with these targets.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM059484-04
Application #
6520045
Study Section
Special Emphasis Panel (ZRG1-MBC-2 (04))
Program Officer
Anderson, James J
Project Start
1999-05-01
Project End
2003-04-30
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
4
Fiscal Year
2002
Total Cost
$296,301
Indirect Cost
Name
University of Illinois at Chicago
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
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Barker, Clive S; Pruss, Birgit M; Matsumura, Philip (2004) Increased motility of Escherichia coli by insertion sequence element integration into the regulatory region of the flhD operon. J Bacteriol 186:7529-37
Pruss, Birgit M; Campbell, John W; Van Dyk, Tina K et al. (2003) FlhD/FlhC is a regulator of anaerobic respiration and the Entner-Doudoroff pathway through induction of the methyl-accepting chemotaxis protein Aer. J Bacteriol 185:534-43
Wang, S; Matsumura, P; Westbrook, E M (2001) Crystallization and preliminary X-ray crystallographic analysis of FlhD/FlhC complex from Escherichia coli. Acta Crystallogr D Biol Crystallogr 57:734-6
Campos, A; Matsumura, P (2001) Extensive alanine scanning reveals protein-protein and protein-DNA interaction surfaces in the global regulator FlhD from Escherichia coli. Mol Microbiol 39:581-94
Pruss, B M; Liu, X; Hendrickson, W et al. (2001) FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions. FEMS Microbiol Lett 197:91-7
Campos, A; Zhang, R G; Alkire, R W et al. (2001) Crystal structure of the global regulator FlhD from Escherichia coli at 1.8 A resolution. Mol Microbiol 39:567-80