Abstract

This is a proposal to develop an integrated genetic resource for investigations of complex traits in the vervet monkey (Cercopithecus aethiops), also known as the African Green monkey. Studies of non-human primates are invaluable for furthering understanding of a wide range of human diseases and traits, especially complex behaviors. The utility of such studies derives from the fact that humans are much more similar to other primates than they are to commonly used mammalian model organisms such as the mouse. The vervet is widely used in biomedical research, particularly for investigation of complex behavioral traits. A captive population at the UCLA-VA Vervet Monkey Research Colony (VMRC) contains a large number of normally reared animals in an extended six-generation pedigree, with most relationships verified by DNA marker typing. Sub-sections of this pedigree will be genotyped to construct two related genetic maps. The first map consists of about 600 human microsatellite markers that cover most of the human genome, and that have already been shown to be polymorphic in vervets under standard genotyping conditions. The relative location of these markers in the vervet genome will be confirmed by multipoint linkage analyses and fluorescence in situ hybridization (FISH). Additional markers will be chosen to minimize the number of large gaps (greater than 15 cM) in the genetic map. A complementary map will consist of up to 2000 single nucleotide polymorphisms (SNPs) drawn from the 3? end of genes. These maps will be used by the scientific community for genetic mapping studies of complex traits in the VMRC pedigree and in vervet populations sited at several other locations. A large insert clone library that we will construct will anchor the genetic maps to the vervet genome and will facilitate the identification of genes that are implicated in predisposition to particular vervet traits. This library will also be useful for investigators who wish to study use of a vervet model to study particular candidate genes identified from investigations of humans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
1R01RR016300-01A1
Application #
6470879
Study Section
Genome Study Section (GNM)
Program Officer
Harding, John D
Project Start
2002-09-01
Project End
2004-08-31
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
1
Fiscal Year
2002
Total Cost
$410,241
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Garcia, Erin I; Emerman, Michael (2018) Recurrent Loss of APOBEC3H Activity during Primate Evolution. J Virol :
Turner, Trudy R; Schmitt, Christopher A; Cramer, Jennifer Danzy et al. (2018) Morphological variation in the genus Chlorocebus: Ecogeographic and anthropogenically mediated variation in body mass, postcranial morphology, and growth. Am J Phys Anthropol 166:682-707
Schmitt, C A; Service, S K; Jasinska, A J et al. (2018) Obesity and obesogenic growth are both highly heritable and modified by diet in a nonhuman primate model, the African green monkey (Chlorocebus aethiops sabaeus). Int J Obes (Lond) 42:765-774
Svardal, Hannes; Jasinska, Anna J; Apetrei, Cristian et al. (2017) Ancient hybridization and strong adaptation to viruses across African vervet monkey populations. Nat Genet 49:1705-1713
Jasinska, Anna J; Zelaya, Ivette; Service, Susan K et al. (2017) Genetic variation and gene expression across multiple tissues and developmental stages in a nonhuman primate. Nat Genet 49:1714-1721
Bailey, Adam L; Lauck, Michael; Sibley, Samuel D et al. (2016) Zoonotic Potential of Simian Arteriviruses. J Virol 90:630-5
Turner, Trudy R; Cramer, Jennifer Danzy; Nisbett, Alexis et al. (2016) A comparison of adult body size between captive and wild vervet monkeys (Chlorocebus aethiops sabaeus) on the island of St. Kitts. Primates 57:211-20
Turner, Trudy R; Coetzer, Willem G; Schmitt, Christopher A et al. (2016) Localized population divergence of vervet monkeys (Chlorocebus spp.) in South Africa: Evidence from mtDNA. Am J Phys Anthropol 159:17-30
Senghore, Madikay; Bayliss, Sion C; Kwambana-Adams, Brenda A et al. (2016) Transmission of Staphylococcus aureus from Humans to Green Monkeys in The Gambia as Revealed by Whole-Genome Sequencing. Appl Environ Microbiol 82:5910-7
Kuokkanen, Satu; Polotsky, Alex J; Chosich, Justin et al. (2016) Corpus luteum as a novel target of weight changes that contribute to impaired female reproductive physiology and function. Syst Biol Reprod Med 62:227-42

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